1hl6

From Proteopedia

(Difference between revisions)

Current revision

A novel mode of RBD-protein recognition in the Y14-mago complex

Structural highlights

1hl6 is a 4 chain structure with sequence from Drosophila melanogaster. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.5Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

RBM8A_DROME Involved in mRNA quality control via the nonsense-mediated mRNA decay (NMD) pathway. Also involved in localization of osk (oskar) mRNA in the posterior pole of oocytes via its interaction with mago.^[1] ^[2]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Y14 and Mago are conserved eukaryotic proteins that associate with spliced mRNAs in the nucleus and remain associated at exon junctions during and after nuclear export. In the cytoplasm, Y14 is involved in mRNA quality control via the nonsense-mediated mRNA decay (NMD) pathway and, together with Mago, is involved in localization of osk (oskar) mRNA. We have determined the crystal structure of the complex between Drosophila melanogaster Y14 and Mago at a resolution of 2.5 A. The structure reveals an atypical mode of protein-protein recognition mediated by an RNA-binding domain (RBD). Instead of binding RNA, the RBD of Y14 engages its RNP1 and RNP2 motifs to bind Mago. Using structure-guided mutagenesis, we show that Mago is also a component of the NMD pathway, and that its association with Y14 is essential for function. Heterodimerization creates a single structural platform that interacts with the NMD machinery via phylogenetically conserved residues.

A novel mode of RBD-protein recognition in the Y14-Mago complex.,Fribourg S, Gatfield D, Izaurralde E, Conti E Nat Struct Biol. 2003 Jun;10(6):433-9. PMID:12730685^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Mohr SE, Dillon ST, Boswell RE. The RNA-binding protein Tsunagi interacts with Mago Nashi to establish polarity and localize oskar mRNA during Drosophila oogenesis. Genes Dev. 2001 Nov 1;15(21):2886-99. PMID:11691839 doi:http://dx.doi.org/10.1101/gad.927001
↑ Fribourg S, Gatfield D, Izaurralde E, Conti E. A novel mode of RBD-protein recognition in the Y14-Mago complex. Nat Struct Biol. 2003 Jun;10(6):433-9. PMID:12730685 doi:10.1038/nsb926
↑ Fribourg S, Gatfield D, Izaurralde E, Conti E. A novel mode of RBD-protein recognition in the Y14-Mago complex. Nat Struct Biol. 2003 Jun;10(6):433-9. PMID:12730685 doi:10.1038/nsb926

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1hl6"

@@ Line 3: / Line 3: @@
 <StructureSection load='1hl6' size='340' side='right'caption='[[1hl6]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[1hl6]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HL6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1HL6 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[1hl6]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HL6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1HL6 FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1hl6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hl6 OCA], [https://pdbe.org/1hl6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1hl6 RCSB], [https://www.ebi.ac.uk/pdbsum/1hl6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1hl6 ProSAT]</span></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1hl6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hl6 OCA], [https://pdbe.org/1hl6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1hl6 RCSB], [https://www.ebi.ac.uk/pdbsum/1hl6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1hl6 ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[https://www.uniprot.org/uniprot/RBM8A_DROME RBM8A_DROME]] Involved in mRNA quality control via the nonsense-mediated mRNA decay (NMD) pathway. Also involved in localization of osk (oskar) mRNA in the posterior pole of oocytes via its interaction with mago.<ref>PMID:11691839</ref> <ref>PMID:12730685</ref>  [[https://www.uniprot.org/uniprot/MGN_DROME MGN_DROME]] Participates in the bidirectional intercellular signaling between the posterior follicle cells and oocyte to establish spatial coordinates that induces axis formation. Complex with tsu is essential for cytoplasmic localization of oskar in the posterior pole of oocytes. Required for the polarization of the oocyte microtubule cytoskeleton.<ref>PMID:8026338</ref> <ref>PMID:9272960</ref> <ref>PMID:9210377</ref> <ref>PMID:12730685</ref>
+[https://www.uniprot.org/uniprot/RBM8A_DROME RBM8A_DROME] Involved in mRNA quality control via the nonsense-mediated mRNA decay (NMD) pathway. Also involved in localization of osk (oskar) mRNA in the posterior pole of oocytes via its interaction with mago.<ref>PMID:11691839</ref> <ref>PMID:12730685</ref>
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 31: / Line 32: @@
 __TOC__
 </StructureSection>
+[[Category: Drosophila melanogaster]]
 [[Category: Large Structures]]
-[[Category: Conti, E]]
+[[Category: Conti E]]
-[[Category: Fribourg, S]]
+[[Category: Fribourg S]]
-[[Category: Gatfield, D]]
+[[Category: Gatfield D]]
-[[Category: Izaurralde, E]]
+[[Category: Izaurralde E]]
-[[Category: Yao, W]]
+[[Category: Yao W]]
-[[Category: Exon-exon junction]]
-[[Category: Nmd]]
-[[Category: Oskar]]
-[[Category: Rdb]]
-[[Category: Rnp]]
-[[Category: Signal protein]]

1hl6

From Proteopedia

Current revision

A novel mode of RBD-protein recognition in the Y14-mago complex

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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