1a4j

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(New page: 200px<br /> <applet load="1a4j" size="450" color="white" frame="true" align="right" spinBox="true" caption="1a4j, resolution 2.1&Aring;" /> '''DIELS ALDER CATALYTI...)
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[[Image:1a4j.gif|left|200px]]<br />
 
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<applet load="1a4j" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1a4j, resolution 2.1&Aring;" />
 
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'''DIELS ALDER CATALYTIC ANTIBODY GERMLINE PRECURSOR'''<br />
 
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==Overview==
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==DIELS ALDER CATALYTIC ANTIBODY GERMLINE PRECURSOR==
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The three-dimensional structure of an antibody (39-A11) that catalyzes a, Diels-Alder reaction has been determined. The structure suggests that the, antibody catalyzes this pericyclic reaction through a combination of, packing and hydrogen-bonding interactions that control the relative, geometries of the bound substrates and electronic distribution in the, dienophile. A single somatic mutation, serine-91 of the light chain to, valine, is largely responsible for the increase in affinity and catalytic, activity of the affinity-matured antibody. Structural and functional, studies of the germ-line precursor suggest that 39-A11 and related, antibodies derive from a family of germ-line genes that have been selected, throughout evolution for the ability of the encoded proteins to form a, polyspecific combining site. Germ line-encoded antibodies of this type, which can rapidly evolve into high-affinity receptors for a broad range of, structures, may help to expand the binding potential associated with the, structural diversity of the primary antibody repertoire.
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<StructureSection load='1a4j' size='340' side='right'caption='[[1a4j]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1a4j]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A4J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1A4J FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1a4j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1a4j OCA], [https://pdbe.org/1a4j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1a4j RCSB], [https://www.ebi.ac.uk/pdbsum/1a4j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1a4j ProSAT]</span></td></tr>
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</table>
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== Disease ==
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[https://www.uniprot.org/uniprot/IGKC_HUMAN IGKC_HUMAN] Defects in IGKC are the cause of immunoglobulin kappa light chain deficiency (IGKCD) [MIM:[https://omim.org/entry/614102 614102]. IGKCD is a disease characterized by the complete absence of immunoglobulin kappa chains.<ref>PMID:3931219</ref>
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== Function ==
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[https://www.uniprot.org/uniprot/IGKC_HUMAN IGKC_HUMAN]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/a4/1a4j_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1a4j ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The three-dimensional structure of an antibody (39-A11) that catalyzes a Diels-Alder reaction has been determined. The structure suggests that the antibody catalyzes this pericyclic reaction through a combination of packing and hydrogen-bonding interactions that control the relative geometries of the bound substrates and electronic distribution in the dienophile. A single somatic mutation, serine-91 of the light chain to valine, is largely responsible for the increase in affinity and catalytic activity of the affinity-matured antibody. Structural and functional studies of the germ-line precursor suggest that 39-A11 and related antibodies derive from a family of germ-line genes that have been selected throughout evolution for the ability of the encoded proteins to form a polyspecific combining site. Germ line-encoded antibodies of this type, which can rapidly evolve into high-affinity receptors for a broad range of structures, may help to expand the binding potential associated with the structural diversity of the primary antibody repertoire.
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==Disease==
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Immunological origins of binding and catalysis in a Diels-Alderase antibody.,Romesberg FE, Spiller B, Schultz PG, Stevens RC Science. 1998 Mar 20;279(5358):1929-33. PMID:9506942<ref>PMID:9506942</ref>
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Known disease associated with this structure: Kappa light chain deficiency OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=147200 147200]]
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==About this Structure==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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1A4J is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1A4J OCA].
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</div>
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<div class="pdbe-citations 1a4j" style="background-color:#fffaf0;"></div>
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==Reference==
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==See Also==
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Immunological origins of binding and catalysis in a Diels-Alderase antibody., Romesberg FE, Spiller B, Schultz PG, Stevens RC, Science. 1998 Mar 20;279(5358):1929-33. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9506942 9506942]
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*[[Monoclonal Antibodies 3D structures|Monoclonal Antibodies 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Mus musculus]]
[[Category: Mus musculus]]
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[[Category: Single protein]]
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[[Category: Romesburg FE]]
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[[Category: Romesburg, F.E.]]
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[[Category: Schultz PG]]
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[[Category: Schultz, P.G.]]
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[[Category: Spiller BW]]
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[[Category: Spiller, B.W.]]
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[[Category: Stevens RC]]
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[[Category: Stevens, R.C.]]
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[[Category: antibody]]
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[[Category: catalytic antibody]]
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[[Category: diels alder]]
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[[Category: germline]]
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[[Category: immunoglobulin]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 15:55:28 2007''
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Current revision

DIELS ALDER CATALYTIC ANTIBODY GERMLINE PRECURSOR

PDB ID 1a4j

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