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6lta

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Crystal Structure of Nonribosomal peptide synthetases (NRPS), FmoA3 (S1046A)

Structural highlights

6lta is a 1 chain structure with sequence from Streptomyces sp. Sp080513GE-23. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.45Å
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

A0A077JG85_9ACTN

Publication Abstract from PubMed

Nonribosomal peptide synthetases (NRPSs) are attractive targets for bioengineering to generate useful peptides . F moA3 is a single modular NRPS composed of heterocyclization (Cy), adenylation (A), and peptidyl carrier protein (PCP) domains. It use s alpha - methyl- l -serine to synthesize a 4-methyloxazoline ring, probably with another Cy domain in the preceding module FmoA2. Here, we determined the head-to-tail homodimeric structures of FmoA3 by X-ray crystallography ( apo -form, with adenylyl-imidodiphosphate and alpha -methyl- l -seryl-AMP) and cryogenic electron microscopy single particle analysis, and performed site-directed mutagenesis experiments. The data revealed that alpha -methyl- l -serine can be accommodated in the active site because of the extra space around Ala688. The Cy domains of FmoA2 and FmoA3 catalyze peptide bond formation and heterocyclization, respectively. FmoA3's Cy domain seems to lose its donor PCP binding activit y. The collective data support a proposed catalytic cycle of FmoA3.

Structural and functional analyses of the tridomain-nonribosomal peptide synthetase FmoA3 for 4-methyloxazoline ring formation.,Katsuyama Y, Sone K, Harada A, Kawai S, Urano N, Adachi N, Moriya T, Kawasaki M, Shin-Ya K, Senda T, Ohnishi Y Angew Chem Int Ed Engl. 2021 Mar 29. doi: 10.1002/anie.202102760. PMID:33783097^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Katsuyama Y, Sone K, Harada A, Kawai S, Urano N, Adachi N, Moriya T, Kawasaki M, Shin-Ya K, Senda T, Ohnishi Y. Structural and functional analyses of the tridomain-nonribosomal peptide synthetase FmoA3 for 4-methyloxazoline ring formation. Angew Chem Int Ed Engl. 2021 Mar 29. doi: 10.1002/anie.202102760. PMID:33783097 doi:http://dx.doi.org/10.1002/anie.202102760

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/6lta"

Categories: Large Structures | Streptomyces sp. Sp080513GE-23 | Harada A | Senda T

@@ Line 3: / Line 3: @@
 <StructureSection load='6lta' size='340' side='right'caption='[[6lta]], [[Resolution|resolution]] 2.45&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[6lta]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_sp._sp080513ge-23 Streptomyces sp. sp080513ge-23]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6LTA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6LTA FirstGlance]. <br>
+<table><tr><td colspan='2'>[[6lta]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_sp._Sp080513GE-23 Streptomyces sp. Sp080513GE-23]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6LTA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6LTA FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AKR:ACRYLIC+ACID'>AKR</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.45&#8491;</td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">fmoA3 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=630397 Streptomyces sp. Sp080513GE-23])</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AKR:ACRYLIC+ACID'>AKR</scene></td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6lta FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6lta OCA], [https://pdbe.org/6lta PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6lta RCSB], [https://www.ebi.ac.uk/pdbsum/6lta PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6lta ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/A0A077JG85_9ACTN A0A077JG85_9ACTN]
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 22: / Line 24: @@
 </StructureSection>
 [[Category: Large Structures]]
-[[Category: Streptomyces sp. sp080513ge-23]]
+[[Category: Streptomyces sp. Sp080513GE-23]]
-[[Category: Harada, A]]
+[[Category: Harada A]]
-[[Category: Senda, T]]
+[[Category: Senda T]]
-[[Category: Biosynthetic protein]]
-[[Category: Jbir-34 and -35]]

6lta

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Current revision

Crystal Structure of Nonribosomal peptide synthetases (NRPS), FmoA3 (S1046A)

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

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