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1e4p

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[[Image:1e4p.gif|left|200px]]
 
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==Structure of the ribozyme substrate hairpin of Neurospora VS RNA. A close look at the cleavage site==
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The line below this paragraph, containing "STRUCTURE_1e4p", creates the "Structure Box" on the page.
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<StructureSection load='1e4p' size='340' side='right'caption='[[1e4p]]' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1e4p]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Neurospora Neurospora]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1E4P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1E4P FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1e4p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1e4p OCA], [https://pdbe.org/1e4p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1e4p RCSB], [https://www.ebi.ac.uk/pdbsum/1e4p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1e4p ProSAT]</span></td></tr>
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{{STRUCTURE_1e4p| PDB=1e4p | SCENE= }}
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The cleavage site of the Neurospora VS RNA ribozyme is located in a separate hairpin domain containing a hexanucleotide internal loop with an A-C mismatch and two adjacent G-A mismatches. The solution structure of the internal loop and helix la of the ribozyme substrate hairpin has been determined by nuclear magnetic resonance (NMR) spectroscopy. The 2 nt in the internal loop, flanking the cleavage site, a guanine and adenine, are involved in two sheared G.A base pairs similar to the magnesium ion-binding site of the hammerhead ribozyme. Adjacent to the tandem G.A base pairs, the adenine and cytidine, which are important for cleavage, form a noncanonical wobble A+-C base pair. The dynamic properties of the internal loop and details of the high-resolution structure support the view that the hairpin structure represents a ground state, which has to undergo a conformational change prior to cleavage. Results of chemical modification and mutagenesis data of the Neurospora VS RNA ribozyme can be explained in context with the present three-dimensional structure.
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'''STRUCTURE OF THE RIBOZYME SUBSTRATE HAIRPIN OF NEUROSPORA VS RNA. A CLOSE LOOK AT THE CLEAVAGE SITE'''
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Structure of the ribozyme substrate hairpin of Neurospora VS RNA: a close look at the cleavage site.,Michiels PJ, Schouten CH, Hilbers CW, Heus HA RNA. 2000 Dec;6(12):1821-32. PMID:11142381<ref>PMID:11142381</ref>
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==Overview==
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The cleavage site of the Neurospora VS RNA ribozyme is located in a separate hairpin domain containing a hexanucleotide internal loop with an A-C mismatch and two adjacent G-A mismatches. The solution structure of the internal loop and helix la of the ribozyme substrate hairpin has been determined by nuclear magnetic resonance (NMR) spectroscopy. The 2 nt in the internal loop, flanking the cleavage site, a guanine and adenine, are involved in two sheared G.A base pairs similar to the magnesium ion-binding site of the hammerhead ribozyme. Adjacent to the tandem G.A base pairs, the adenine and cytidine, which are important for cleavage, form a noncanonical wobble A+-C base pair. The dynamic properties of the internal loop and details of the high-resolution structure support the view that the hairpin structure represents a ground state, which has to undergo a conformational change prior to cleavage. Results of chemical modification and mutagenesis data of the Neurospora VS RNA ribozyme can be explained in context with the present three-dimensional structure.
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==About this Structure==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1E4P OCA].
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</div>
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<div class="pdbe-citations 1e4p" style="background-color:#fffaf0;"></div>
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==Reference==
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==See Also==
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Structure of the ribozyme substrate hairpin of Neurospora VS RNA: a close look at the cleavage site., Michiels PJ, Schouten CH, Hilbers CW, Heus HA, RNA. 2000 Dec;6(12):1821-32. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11142381 11142381]
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*[[Ribozyme 3D structures|Ribozyme 3D structures]]
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[[Category: Heus, H A.]]
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== References ==
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[[Category: Hilbers, C W.]]
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<references/>
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[[Category: Michiels, P J.A.]]
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__TOC__
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[[Category: Schouten, C H.J.]]
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</StructureSection>
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[[Category: Rna ribozyme]]
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[[Category: Large Structures]]
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[[Category: Substrate hairpin]]
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[[Category: Neurospora]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 14:39:32 2008''
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[[Category: Heus HA]]
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[[Category: Hilbers CW]]
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[[Category: Michiels PJA]]
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[[Category: Schouten CHJ]]

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Structure of the ribozyme substrate hairpin of Neurospora VS RNA. A close look at the cleavage site

PDB ID 1e4p

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