6lbh
From Proteopedia
(Difference between revisions)
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| - | ==== | + | ==Cryo-EM structure of the MgtE Mg2+ channel under Mg2+-free conditions== |
| - | <StructureSection load='6lbh' size='340' side='right'caption='[[6lbh]]' scene=''> | + | <StructureSection load='6lbh' size='340' side='right'caption='[[6lbh]], [[Resolution|resolution]] 3.70Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6lbh]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus] and [https://en.wikipedia.org/wiki/Thermus_thermophilus_HB8 Thermus thermophilus HB8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6LBH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6LBH FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6lbh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6lbh OCA], [https://pdbe.org/6lbh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6lbh RCSB], [https://www.ebi.ac.uk/pdbsum/6lbh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6lbh ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.7Å</td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6lbh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6lbh OCA], [https://pdbe.org/6lbh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6lbh RCSB], [https://www.ebi.ac.uk/pdbsum/6lbh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6lbh ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/MGTE_THET8 MGTE_THET8] Acts as a highly selective magnesium channel.<ref>PMID:17700703</ref> <ref>PMID:19798051</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | MgtE is a Mg2+ channel conserved in organisms ranging from prokaryotes to eukaryotes, including humans, and plays an important role in Mg2+ homeostasis. The previously determined MgtE structures in the Mg2+-bound, closed-state, and structure-based functional analyses of MgtE revealed that the binding of Mg2+ ions to the MgtE cytoplasmic domain induces channel inactivation to maintain Mg2+ homeostasis. There are no structures of the transmembrane (TM) domain for MgtE in Mg2+-free conditions, and the pore-opening mechanism has thus remained unclear. Here, we determined the cryo-electron microscopy (cryo-EM) structure of the MgtE-Fab complex in the absence of Mg2+ ions. The Mg2+-free MgtE TM domain structure and its comparison with the Mg2+-bound, closed-state structure, together with functional analyses, showed the Mg2+-dependent pore opening of MgtE on the cytoplasmic side and revealed the kink motions of the TM2 and TM5 helices at the glycine residues, which are important for channel activity. Overall, our work provides structure-based mechanistic insights into the channel gating of MgtE. | ||
| + | |||
| + | The structure of MgtE in the absence of magnesium provides new insights into channel gating.,Jin F, Sun M, Fujii T, Yamada Y, Wang J, Maturana AD, Wada M, Su S, Ma J, Takeda H, Kusakizako T, Tomita A, Nakada-Nakura Y, Liu K, Uemura T, Nomura Y, Nomura N, Ito K, Nureki O, Namba K, Iwata S, Yu Y, Hattori M PLoS Biol. 2021 Apr 27;19(4):e3001231. doi: 10.1371/journal.pbio.3001231. , eCollection 2021 Apr. PMID:33905418<ref>PMID:33905418</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6lbh" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: | + | [[Category: Mus musculus]] |
| + | [[Category: Thermus thermophilus HB8]] | ||
| + | [[Category: Hattori M]] | ||
| + | [[Category: Jin F]] | ||
Current revision
Cryo-EM structure of the MgtE Mg2+ channel under Mg2+-free conditions
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