1a9n

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(New page: 200px<br /> <applet load="1a9n" size="450" color="white" frame="true" align="right" spinBox="true" caption="1a9n, resolution 2.38&Aring;" /> '''CRYSTAL STRUCTURE O...)
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[[Image:1a9n.gif|left|200px]]<br />
 
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<applet load="1a9n" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1a9n, resolution 2.38&Aring;" />
 
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'''CRYSTAL STRUCTURE OF THE SPLICEOSOMAL U2B''-U2A' PROTEIN COMPLEX BOUND TO A FRAGMENT OF U2 SMALL NUCLEAR RNA'''<br />
 
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==Overview==
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==CRYSTAL STRUCTURE OF THE SPLICEOSOMAL U2B''-U2A' PROTEIN COMPLEX BOUND TO A FRAGMENT OF U2 SMALL NUCLEAR RNA==
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We have determined the crystal structure at 2.4 A resolution of a ternary, complex between the spliceosomal U2B"/U2A' protein complex and, hairpin-loop IV of U2 small nuclear RNA. Unlike its close homologue the, U1A protein, U2B" binds to its cognate RNA only in the presence of U2A', which contains leucine-rich repeats in its sequence. The concave surface, of a parallel beta-sheet within the leucine-rich-repeat region of U2A', interacts with the ribonucleoprotein domain of U2B" on the surface, opposite its RNA-binding surface. The basic carboxy-terminal region of, U2A' interacts with the RNA stem. The crystal structure reveals how, protein-protein interaction regulates RNA-binding specificity, and how, replacing only a few key residues allows the U2B" and U1A proteins to, discriminate between their cognate RNA hairpins by forming alternative, networks of interactions.
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<StructureSection load='1a9n' size='340' side='right'caption='[[1a9n]], [[Resolution|resolution]] 2.38&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1a9n]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A9N OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1A9N FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.38&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1a9n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1a9n OCA], [https://pdbe.org/1a9n PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1a9n RCSB], [https://www.ebi.ac.uk/pdbsum/1a9n PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1a9n ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RU2B_HUMAN RU2B_HUMAN] Involved in pre-mRNA splicing. This protein is associated with snRNP U2. It binds stem loop IV of U2 snRNA only in presence of the U2A' protein.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/a9/1a9n_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1a9n ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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We have determined the crystal structure at 2.4 A resolution of a ternary complex between the spliceosomal U2B"/U2A' protein complex and hairpin-loop IV of U2 small nuclear RNA. Unlike its close homologue the U1A protein, U2B" binds to its cognate RNA only in the presence of U2A', which contains leucine-rich repeats in its sequence. The concave surface of a parallel beta-sheet within the leucine-rich-repeat region of U2A' interacts with the ribonucleoprotein domain of U2B" on the surface opposite its RNA-binding surface. The basic carboxy-terminal region of U2A' interacts with the RNA stem. The crystal structure reveals how protein-protein interaction regulates RNA-binding specificity, and how replacing only a few key residues allows the U2B" and U1A proteins to discriminate between their cognate RNA hairpins by forming alternative networks of interactions.
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==About this Structure==
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Crystal structure of the spliceosomal U2B"-U2A' protein complex bound to a fragment of U2 small nuclear RNA.,Price SR, Evans PR, Nagai K Nature. 1998 Aug 13;394(6694):645-50. PMID:9716128<ref>PMID:9716128</ref>
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1A9N is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1A9N OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Crystal structure of the spliceosomal U2B"-U2A' protein complex bound to a fragment of U2 small nuclear RNA., Price SR, Evans PR, Nagai K, Nature. 1998 Aug 13;394(6694):645-50. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9716128 9716128]
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</div>
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<div class="pdbe-citations 1a9n" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Protein complex]]
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[[Category: Large Structures]]
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[[Category: Evans, P.R.]]
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[[Category: Evans PR]]
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[[Category: Nagai, K.]]
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[[Category: Nagai K]]
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[[Category: Price, S.R.]]
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[[Category: Price SR]]
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[[Category: complex (nuclear protein/rna)]]
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[[Category: ribonucleoprotein]]
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[[Category: rna]]
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[[Category: snrnp]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 15:57:03 2007''
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Current revision

CRYSTAL STRUCTURE OF THE SPLICEOSOMAL U2B-U2A' PROTEIN COMPLEX BOUND TO A FRAGMENT OF U2 SMALL NUCLEAR RNA

PDB ID 1a9n

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