2kss

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (06:48, 1 May 2024) (edit) (undo)
 
(One intermediate revision not shown.)
Line 1: Line 1:
==NMR structure of Myxococcus xanthus antirepressor CarS1==
==NMR structure of Myxococcus xanthus antirepressor CarS1==
-
<StructureSection load='2kss' size='340' side='right'caption='[[2kss]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
+
<StructureSection load='2kss' size='340' side='right'caption='[[2kss]]' scene=''>
== Structural highlights ==
== Structural highlights ==
-
<table><tr><td colspan='2'>[[2kss]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_25232 Atcc 25232]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KSS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2KSS FirstGlance]. <br>
+
<table><tr><td colspan='2'>[[2kss]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Myxococcus_xanthus Myxococcus xanthus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KSS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2KSS FirstGlance]. <br>
-
</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">carS ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=34 ATCC 25232])</td></tr>
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2kss FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kss OCA], [https://pdbe.org/2kss PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2kss RCSB], [https://www.ebi.ac.uk/pdbsum/2kss PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2kss ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2kss FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kss OCA], [https://pdbe.org/2kss PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2kss RCSB], [https://www.ebi.ac.uk/pdbsum/2kss PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2kss ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
-
[[https://www.uniprot.org/uniprot/CARS_MYXXA CARS_MYXXA]] Involved in carotenoid biosynthesis. Antagonizes the transcriptional repressor proteins CarA and CarH by preventing their binding to DNA. Can also dissociate preformed CarA-DNA complexes. Does not bind DNA.<ref>PMID:11748235</ref> <ref>PMID:18315685</ref>
+
[https://www.uniprot.org/uniprot/CARS_MYXXA CARS_MYXXA] Involved in carotenoid biosynthesis. Antagonizes the transcriptional repressor proteins CarA and CarH by preventing their binding to DNA. Can also dissociate preformed CarA-DNA complexes. Does not bind DNA.<ref>PMID:11748235</ref> <ref>PMID:18315685</ref>
-
<div style="background-color:#fffaf0;">
+
-
== Publication Abstract from PubMed ==
+
-
Direct targeting of critical DNA-binding elements of a repressor by its cognate antirepressor is an effective means to sequester the repressor and remove a transcription initiation block. Structural descriptions for this, though often proposed for bacterial and phage repressor-antirepressor systems, are unavailable. Here, we describe the structural and functional basis of how the Myxococcus xanthus CarS antirepressor recognizes and neutralizes its cognate repressors to turn on a photo-inducible promoter. CarA and CarH repress the carB operon in the dark. CarS, produced in the light, physically interacts with the MerR-type winged-helix DNA-binding domain of these repressors leading to activation of carB. The NMR structure of CarS1, a functional CarS variant, reveals a five-stranded, antiparallel beta-sheet fold resembling SH3 domains, protein-protein interaction modules prevalent in eukaryotes but rare in prokaryotes. NMR studies and analysis of site-directed mutants in vivo and in vitro unveil a solvent-exposed hydrophobic pocket lined by acidic residues in CarS, where the CarA DNA recognition helix docks with high affinity in an atypical ligand-recognition mode for SH3 domains. Our findings uncover an unprecedented use of the SH3 domain-like fold for protein-protein recognition whereby an antirepressor mimics operator DNA in sequestering the repressor DNA recognition helix to activate transcription.
+
-
 
+
-
A bacterial antirepressor with SH3 domain topology mimics operator DNA in sequestering the repressor DNA recognition helix.,Leon E, Navarro-Aviles G, Santiveri CM, Flores-Flores C, Rico M, Gonzalez C, Murillo FJ, Elias-Arnanz M, Jimenez MA, Padmanabhan S Nucleic Acids Res. 2010 Aug;38(15):5226-41. Epub 2010 Apr 21. PMID:20410074<ref>PMID:20410074</ref>
+
-
 
+
-
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+
-
</div>
+
-
<div class="pdbe-citations 2kss" style="background-color:#fffaf0;"></div>
+
== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
-
[[Category: Atcc 25232]]
 
[[Category: Large Structures]]
[[Category: Large Structures]]
-
[[Category: Elias-Arnanz, M]]
+
[[Category: Myxococcus xanthus]]
-
[[Category: Gonzalez, C]]
+
[[Category: Elias-Arnanz M]]
-
[[Category: Jimenez, M]]
+
[[Category: Gonzalez C]]
-
[[Category: Leon, E]]
+
[[Category: Jimenez M]]
-
[[Category: Navarro-Aviles, G]]
+
[[Category: Leon E]]
-
[[Category: Padmanabhan, S]]
+
[[Category: Navarro-Aviles G]]
-
[[Category: Activator]]
+
[[Category: Padmanabhan S]]
-
[[Category: Antirepressor]]
+
-
[[Category: Carotenoid biosynthesis]]
+
-
[[Category: Protein]]
+
-
[[Category: Transcription]]
+
-
[[Category: Transcription regulation]]
+
-
[[Category: Transcription regulator]]
+

Current revision

NMR structure of Myxococcus xanthus antirepressor CarS1

PDB ID 2kss

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2kss"
Personal tools