2l0t

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==Solution structure of the complex of ubiquitin and the VHS domain of Stam2==
==Solution structure of the complex of ubiquitin and the VHS domain of Stam2==
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<StructureSection load='2l0t' size='340' side='right'caption='[[2l0t]], [[NMR_Ensembles_of_Models | 10 NMR models]]' scene=''>
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<StructureSection load='2l0t' size='340' side='right'caption='[[2l0t]]' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[2l0t]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2L0T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2L0T FirstGlance]. <br>
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<table><tr><td colspan='2'>[[2l0t]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2L0T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2L0T FirstGlance]. <br>
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</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1d3z|1d3z]], [[1x5b|1x5b]]</div></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">STAM2, HBP ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2l0t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2l0t OCA], [https://pdbe.org/2l0t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2l0t RCSB], [https://www.ebi.ac.uk/pdbsum/2l0t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2l0t ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2l0t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2l0t OCA], [https://pdbe.org/2l0t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2l0t RCSB], [https://www.ebi.ac.uk/pdbsum/2l0t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2l0t ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
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[[https://www.uniprot.org/uniprot/STAM2_HUMAN STAM2_HUMAN]] Involved in intracellular signal transduction mediated by cytokines and growth factors. Upon IL-2 and GM-CSL stimulation, it plays a role in signaling leading to DNA synthesis and MYC induction. May also play a role in T-cell development. Involved in down-regulation of receptor tyrosine kinase via multivesicular body (MVBs) when complexed with HGS (ESCRT-0 complex). The ESCRT-0 complex binds ubiquitin and acts as sorting machinery that recognizes ubiquitinated receptors and transfers them to further sequential lysosomal sorting/trafficking processes (By similarity).
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[https://www.uniprot.org/uniprot/STAM2_HUMAN STAM2_HUMAN] Involved in intracellular signal transduction mediated by cytokines and growth factors. Upon IL-2 and GM-CSL stimulation, it plays a role in signaling leading to DNA synthesis and MYC induction. May also play a role in T-cell development. Involved in down-regulation of receptor tyrosine kinase via multivesicular body (MVBs) when complexed with HGS (ESCRT-0 complex). The ESCRT-0 complex binds ubiquitin and acts as sorting machinery that recognizes ubiquitinated receptors and transfers them to further sequential lysosomal sorting/trafficking processes (By similarity).
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The VHS domain of the Stam2 protein is a ubiquitin binding domain involved in the recognition of ubiquitinated proteins committed to lysosomal degradation. Among all VHS domains, the VHS domain of Stam proteins is the strongest binder to monoubiqiuitin and exhibits preferences for K63-linked chains. In the present paper, we report the solution NMR structure of the Stam2-VHS domain in complex with monoubiquitin by means of chemical shift perturbations, spin relaxation, and paramagnetic relaxation enhancements. We also characterize the interaction of Stam2-VHS with K48- and K63-linked diubiquitin chains and report the first evidence that VHS binds differently to these two chains. Our data reveal that VHS enters the hydrophobic pocket of K48-linked diubiquitin and binds the two ubiquitin subunits with different affinities. In contrast, VHS interacts with K63-linked diubiquitin in a mode similar to its interaction with monoubiquitin. We also suggest possible structural models for both K48- and K63-linked diubiquitin in interaction with VHS. Our results, which demonstrate a different mode of binding of VHS for K48- and K63-linked diubiquitin, may explain the preference of VHS for K63- over K48-linked diubiquitin chains and monoubiquitin.
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NMR Reveals a Different Mode of Binding of the Stam2 VHS Domain to Ubiquitin and Diubiquitin .,Lange A, Hoeller D, Wienk H, Marcillat O, Lancelin JM, Walker O Biochemistry. 2010 Dec 13. PMID:21121635<ref>PMID:21121635</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2l0t" style="background-color:#fffaf0;"></div>
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==See Also==
==See Also==
*[[3D structures of ubiquitin|3D structures of ubiquitin]]
*[[3D structures of ubiquitin|3D structures of ubiquitin]]
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== References ==
 
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<references/>
 
__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Human]]
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[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Hoeller, D]]
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[[Category: Hoeller D]]
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[[Category: Lancelin, J]]
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[[Category: Lancelin J]]
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[[Category: Lange, A]]
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[[Category: Lange A]]
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[[Category: Marcillat, O]]
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[[Category: Marcillat O]]
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[[Category: Walker, O]]
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[[Category: Walker O]]
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[[Category: Wienk, H]]
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[[Category: Wienk H]]
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[[Category: Endosome]]
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[[Category: Protein transport]]
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[[Category: Stam2]]
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[[Category: Transport]]
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[[Category: Ubiquitin]]
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[[Category: Vh]]
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Current revision

Solution structure of the complex of ubiquitin and the VHS domain of Stam2

PDB ID 2l0t

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