2l9y

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==Solution structure of the MoCVNH-LysM module from the rice blast fungus Magnaporthe oryzae protein (MGG_03307)==
==Solution structure of the MoCVNH-LysM module from the rice blast fungus Magnaporthe oryzae protein (MGG_03307)==
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<StructureSection load='2l9y' size='340' side='right'caption='[[2l9y]], [[NMR_Ensembles_of_Models | 25 NMR models]]' scene=''>
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<StructureSection load='2l9y' size='340' side='right'caption='[[2l9y]]' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[2l9y]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mago7 Mago7]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2L9Y OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2L9Y FirstGlance]. <br>
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<table><tr><td colspan='2'>[[2l9y]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pyricularia_oryzae_70-15 Pyricularia oryzae 70-15]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2L9Y OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2L9Y FirstGlance]. <br>
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</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">MGG_03307 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=242507 MAGO7])</td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2l9y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2l9y OCA], [https://pdbe.org/2l9y PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2l9y RCSB], [https://www.ebi.ac.uk/pdbsum/2l9y PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2l9y ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2l9y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2l9y OCA], [https://pdbe.org/2l9y PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2l9y RCSB], [https://www.ebi.ac.uk/pdbsum/2l9y PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2l9y ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/G4N991_MAGO7 G4N991_MAGO7]
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The rice blast fungus Magnaporthe oryzae's genome encodes a hypothetical protein (MGG_03307) containing a type III CVNH lectin, in which a LysM domain is inserted between individual repeats of a single CVNH domain. At present, no structural or ligand binding data are available for any type III CVNH and functional studies in natural source organisms are scarce. Here, we report NMR solution structure and functional data on MGG_03307. The structure of the CVNH/LysM module revealed that intact and functionally competent CVNH and LysM domains are present. Using NMR titrations, carbohydrate specificities for both domains were determined, and it was found that each domain behaves as an isolated unit without any interdomain communication. Furthermore, live-cell imaging revealed a predominant localization of MGG_03307 within the appressorium, the specialized fungal cell for gaining entry into rice tissue. Our results suggest that MGG_03307 plays a role in the early stages of plant infection.
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Structure-Function Analysis of a CVNH-LysM Lectin Expressed during Plant Infection by the Rice Blast Fungus Magnaporthe oryzae.,Koharudin LM, Viscomi AR, Montanini B, Kershaw MJ, Talbot NJ, Ottonello S, Gronenborn AM Structure. 2011 May 11;19(5):662-74. PMID:21565701<ref>PMID:21565701</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2l9y" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Mago7]]
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[[Category: Pyricularia oryzae 70-15]]
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[[Category: Gronenborn, A M]]
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[[Category: Gronenborn AM]]
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[[Category: Kershaw, M J]]
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[[Category: Kershaw MJ]]
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[[Category: Koharudin, L M.I]]
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[[Category: Koharudin LMI]]
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[[Category: Montanini, B]]
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[[Category: Montanini B]]
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[[Category: Ottonello, S]]
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[[Category: Ottonello S]]
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[[Category: Talbot, N J]]
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[[Category: Talbot NJ]]
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[[Category: Viscomi, A R]]
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[[Category: Viscomi AR]]
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[[Category: Carbohydrate]]
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[[Category: Cvnh]]
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[[Category: Lectin]]
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[[Category: Sugar binding protein]]
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Current revision

Solution structure of the MoCVNH-LysM module from the rice blast fungus Magnaporthe oryzae protein (MGG_03307)

PDB ID 2l9y

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