7lun
From Proteopedia
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==Human PARP14 (ARTD8), catalytic fragment in complex with RBN011980== | ==Human PARP14 (ARTD8), catalytic fragment in complex with RBN011980== | ||
| - | <StructureSection load='7lun' size='340' side='right'caption='[[7lun]]' scene=''> | + | <StructureSection load='7lun' size='340' side='right'caption='[[7lun]], [[Resolution|resolution]] 2.57Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7LUN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7LUN FirstGlance]. <br> | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7LUN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7LUN FirstGlance]. <br> | ||
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7lun FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7lun OCA], [https://pdbe.org/7lun PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7lun RCSB], [https://www.ebi.ac.uk/pdbsum/7lun PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7lun ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.57Å</td></tr> |
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=YFG:7-(cyclopentylamino)-5-fluoro-2-{[(trans-4-hydroxycyclohexyl)sulfanyl]methyl}quinazolin-4(3H)-one'>YFG</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7lun FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7lun OCA], [https://pdbe.org/7lun PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7lun RCSB], [https://www.ebi.ac.uk/pdbsum/7lun PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7lun ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | PARP14 is an interferon-stimulated gene that is overexpressed in multiple tumor types, influencing pro-tumor macrophage polarization as well as suppressing the antitumor inflammation response by modulating IFN-gamma and IL-4 signaling. PARP14 is a 203 kDa protein that possesses a catalytic domain responsible for the transfer of mono-ADP-ribose to its substrates. PARP14 also contains three macrodomains and a WWE domain which are binding modules for mono-ADP-ribose and poly-ADP-ribose, respectively, in addition to two RNA recognition motifs. Catalytic inhibitors of PARP14 have been shown to reverse IL-4 driven pro-tumor gene expression in macrophages, however it is not clear what roles the non-enzymatic biomolecular recognition motifs play in PARP14-driven immunology and inflammation. To further understand this, we have discovered a heterobifunctional small molecule designed based on a catalytic inhibitor of PARP14 that binds in the enzyme's NAD + -binding site and recruits the E3 ligase cereblon to ubiquitinate it and selectively target it for degradation. | ||
| + | |||
| + | Targeted Degradation of PARP14 Using a Heterobifunctional Small Molecule.,Wigle T, Ren Y, Molina JR, Blackwell DJ, Schenkel LB, Swinger KK, Kuplast-Barr K, Majer CR, Church WD, Lu AZ, Mo J, Abo R, Cheung A, Dorsey BW, Niepel M, Perl NR, Vasbinder MM, Keilhack H, Kuntz KW Chembiochem. 2021 Apr 10. doi: 10.1002/cbic.202100047. PMID:33838082<ref>PMID:33838082</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7lun" style="background-color:#fffaf0;"></div> | ||
| + | |||
| + | ==See Also== | ||
| + | *[[Poly(ADP-ribose) polymerase 3D structures|Poly(ADP-ribose) polymerase 3D structures]] | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
Current revision
Human PARP14 (ARTD8), catalytic fragment in complex with RBN011980
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Categories: Large Structures | Church WD | Dorsey BW | Kuntz KW | Perl NR | Schenkel LB | Swinger KK | Vasbinder MM | Wigle TJ
