1kem

From Proteopedia

(Difference between revisions)

Current revision

CATALYTIC ANTIBODY 28B4 FAB FRAGMENT

Structural highlights

1kem is a 2 chain structure with sequence from Mus musculus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.2Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

IGHG1_MOUSE

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The x-ray crystal structures of the sulfide oxidase antibody 28B4 and of antibody 28B4 complexed with hapten have been solved at 2.2-angstrom and 1.9-angstrom resolution, respectively. To our knowledge, these structures are the highest resolution catalytic antibody structures to date and provide insight into the molecular mechanism of this antibody-catalyzed monooxygenation reaction. Specifically, the data suggest that entropic restriction plays a fundamental role in catalysis through the precise alignment of the thioether substrate and oxidant. The antibody active site also stabilizes developing charge on both sulfur and periodate in the transition state via cation-pi and electrostatic interactions, respectively. In addition to demonstrating that the active site of antibody 28B4 does indeed reflect the mechanistic information programmed in the aminophosphonic acid hapten, these high-resolution structures provide a basis for enhancing turnover rates through mutagenesis and improved hapten design.

Insights into antibody catalysis: structure of an oxygenation catalyst at 1.9-angstrom resolution.,Hsieh-Wilson LC, Schultz PG, Stevens RC Proc Natl Acad Sci U S A. 1996 May 28;93(11):5363-7. PMID:8643580^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Hsieh-Wilson LC, Schultz PG, Stevens RC. Insights into antibody catalysis: structure of an oxygenation catalyst at 1.9-angstrom resolution. Proc Natl Acad Sci U S A. 1996 May 28;93(11):5363-7. PMID:8643580

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1kem"

Categories: Large Structures | Mus musculus | Hsieh-Wilson LC | Schultz PG | Stevens RC

@@ Line 3: / Line 3: @@
 <StructureSection load='1kem' size='340' side='right'caption='[[1kem]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[1kem]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KEM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1KEM FirstGlance]. <br>
+<table><tr><td colspan='2'>[[1kem]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KEM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1KEM FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1kem FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kem OCA], [https://pdbe.org/1kem PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1kem RCSB], [https://www.ebi.ac.uk/pdbsum/1kem PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1kem ProSAT]</span></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1kem FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kem OCA], [https://pdbe.org/1kem PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1kem RCSB], [https://www.ebi.ac.uk/pdbsum/1kem PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1kem ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/IGHG1_MOUSE IGHG1_MOUSE]
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 11: / Line 14: @@
   <jmolCheckbox>
     <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ke/1kem_consurf.spt"</scriptWhenChecked>
-    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
     <text>to colour the structure by Evolutionary Conservation</text>
   </jmolCheckbox>
@@ Line 33: / Line 36: @@
 </StructureSection>
 [[Category: Large Structures]]
-[[Category: Lk3 transgenic mice]]
+[[Category: Mus musculus]]
-[[Category: Hsieh-Wilson, L C]]
+[[Category: Hsieh-Wilson LC]]
-[[Category: Schultz, P G]]
+[[Category: Schultz PG]]
-[[Category: Stevens, R C]]
+[[Category: Stevens RC]]
-[[Category: Catalytic antibody]]
-[[Category: Fab]]
-[[Category: Immunoglobulin]]
-[[Category: Monooxygenase]]
-[[Category: Oxygenation]]
-[[Category: Sulfide oxidation]]

1kem

From Proteopedia

Current revision

CATALYTIC ANTIBODY 28B4 FAB FRAGMENT

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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