1knv

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<StructureSection load='1knv' size='340' side='right'caption='[[1knv]], [[Resolution|resolution]] 2.17&Aring;' scene=''>
<StructureSection load='1knv' size='340' side='right'caption='[[1knv]], [[Resolution|resolution]] 2.17&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[1knv]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_12980 Atcc 12980]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KNV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1KNV FirstGlance]. <br>
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<table><tr><td colspan='2'>[[1knv]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Geobacillus_stearothermophilus Geobacillus stearothermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KNV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1KNV FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.17&#8491;</td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1cfr|1cfr]]</div></td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Type_II_site-specific_deoxyribonuclease Type II site-specific deoxyribonuclease], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.21.4 3.1.21.4] </span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1knv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1knv OCA], [https://pdbe.org/1knv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1knv RCSB], [https://www.ebi.ac.uk/pdbsum/1knv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1knv ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1knv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1knv OCA], [https://pdbe.org/1knv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1knv RCSB], [https://www.ebi.ac.uk/pdbsum/1knv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1knv ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/Q8RT53_GEOSE Q8RT53_GEOSE]
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Crystal structures of Type II restriction endonucleases demonstrate a conserved common core and active site residues but diverse structural elements involved in DNA sequence discrimination. Comparative structural analysis of restriction enzymes recognizing the same nucleotide sequence might therefore contribute to our understanding of the structural diversity of specificity determinants within restriction enzymes. We have solved the crystal structure of the Bacillus stearothermophilus restriction endonuclease Bse634I by the multiple isomorphous replacement technique to 2.17 A resolution. Bse634I is an isoschisomer of the Cfr10I restriction enzyme whose crystal structure has been reported previously. Comparative structural analysis of the first pair of isoschisomeric enzymes revealed conserved structural determinants of sequence recognition and catalysis. However, conformations of the N-terminal subdomains differed between Bse634I/Cfr10I, suggesting a rigid body movement that might couple DNA recognition and catalysis. Structural similarities extend to the quaternary structure level: crystal contacts suggest that Bse634I similarly to Cfr10I is arranged as a tetramer. Kinetic analysis reveals that Bse634I is able to interact simultaneously with two recognition sites supporting the tetrameric architecture of the protein. Thus, restriction enzymes Bse634I, Cfr10I and NgoMIV, recognizing overlapping nucleotide sequences, exhibit a conserved tetrameric architecture that is of functional importance.
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Crystal structure of the Bse634I restriction endonuclease: comparison of two enzymes recognizing the same DNA sequence.,Grazulis S, Deibert M, Rimseliene R, Skirgaila R, Sasnauskas G, Lagunavicius A, Repin V, Urbanke C, Huber R, Siksnys V Nucleic Acids Res. 2002 Feb 15;30(4):876-85. PMID:11842098<ref>PMID:11842098</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 1knv" style="background-color:#fffaf0;"></div>
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==See Also==
==See Also==
*[[Endonuclease 3D structures|Endonuclease 3D structures]]
*[[Endonuclease 3D structures|Endonuclease 3D structures]]
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== References ==
 
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<references/>
 
__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Atcc 12980]]
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[[Category: Geobacillus stearothermophilus]]
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Type II site-specific deoxyribonuclease]]
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[[Category: Deibert M]]
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[[Category: Deibert, M]]
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[[Category: Grazulis S]]
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[[Category: Grazulis, S]]
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[[Category: Huber R]]
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[[Category: Huber, R]]
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[[Category: Lagunavicius A]]
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[[Category: Lagunavicius, A]]
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[[Category: Repin V]]
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[[Category: Repin, V]]
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[[Category: Rimseliene R]]
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[[Category: Rimseliene, R]]
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[[Category: Sasnauskas G]]
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[[Category: Sasnauskas, G]]
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[[Category: Siksnys V]]
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[[Category: Siksnys, V]]
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[[Category: Skirgaila R]]
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[[Category: Skirgaila, R]]
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[[Category: Urbanke C]]
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[[Category: Urbanke, C]]
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[[Category: Apo-enzyme]]
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[[Category: Hydrolase]]
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[[Category: Restriction endonuclease]]
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Current revision

Bse634I restriction endonuclease

PDB ID 1knv

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Proteopedia Page Contributors and Editors (what is this?)

OCA

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