6wq1
From Proteopedia
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==Eukaryotic LanCL2 protein== | ==Eukaryotic LanCL2 protein== | ||
| - | <StructureSection load='6wq1' size='340' side='right'caption='[[6wq1]]' scene=''> | + | <StructureSection load='6wq1' size='340' side='right'caption='[[6wq1]], [[Resolution|resolution]] 2.29Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6WQ1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6WQ1 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6wq1]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6WQ1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6WQ1 FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6wq1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6wq1 OCA], [https://pdbe.org/6wq1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6wq1 RCSB], [https://www.ebi.ac.uk/pdbsum/6wq1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6wq1 ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.295Å</td></tr> |
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6wq1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6wq1 OCA], [https://pdbe.org/6wq1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6wq1 RCSB], [https://www.ebi.ac.uk/pdbsum/6wq1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6wq1 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/LANC2_HUMAN LANC2_HUMAN] Necessary for abscisic acid (ABA) binding on the cell membrane and activation of the ABA signaling pathway in granulocytes.<ref>PMID:19667068</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Enzyme-mediated damage repair or mitigation, while common for nucleic acids, is rare for proteins. Examples of protein damage are elimination of phosphorylated Ser/Thr to dehydroalanine/dehydrobutyrine (Dha/Dhb) in pathogenesis and aging. Bacterial LanC enzymes use Dha/Dhb to form carbon-sulfur linkages in antimicrobial peptides, but the functions of eukaryotic LanC-like (LanCL) counterparts are unknown. We show that LanCLs catalyze the addition of glutathione to Dha/Dhb in proteins, driving irreversible C-glutathionylation. Chemo-enzymatic methods were developed to site-selectively incorporate Dha/Dhb at phospho-regulated sites in kinases. In human MAPK-MEK1, such "elimination damage" generated aberrantly activated kinases, which were deactivated by LanCL-mediated C-glutathionylation. Surveys of endogenous proteins bearing damage from elimination (the eliminylome) also suggest it is a source of electrophilic reactivity. LanCLs thus remove these reactive electrophiles and their potentially dysregulatory effects from the proteome. As knockout of LanCL in mice can result in premature death, repair of this kind of protein damage appears important physiologically. | ||
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| + | LanCLs add glutathione to dehydroamino acids generated at phosphorylated sites in the proteome.,Lai KY, Galan SRG, Zeng Y, Zhou TH, He C, Raj R, Riedl J, Liu S, Chooi KP, Garg N, Zeng M, Jones LH, Hutchings GJ, Mohammed S, Nair SK, Chen J, Davis BG, van der Donk WA Cell. 2021 May 13;184(10):2680-2695.e26. doi: 10.1016/j.cell.2021.04.001. Epub, 2021 Apr 30. PMID:33932340<ref>PMID:33932340</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6wq1" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Homo sapiens]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Garg N]] | [[Category: Garg N]] | ||
[[Category: Nair SK]] | [[Category: Nair SK]] | ||
Current revision
Eukaryotic LanCL2 protein
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