4lun

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<StructureSection load='4lun' size='340' side='right'caption='[[4lun]], [[Resolution|resolution]] 1.64&Aring;' scene=''>
<StructureSection load='4lun' size='340' side='right'caption='[[4lun]], [[Resolution|resolution]] 1.64&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[4lun]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4LUN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4LUN FirstGlance]. <br>
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<table><tr><td colspan='2'>[[4lun]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4LUN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4LUN FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.641&#8491;</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">IFS1, NMD2, SUA1, UPF2, YHR077C ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast])</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4lun FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4lun OCA], [https://pdbe.org/4lun PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4lun RCSB], [https://www.ebi.ac.uk/pdbsum/4lun PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4lun ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4lun FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4lun OCA], [https://pdbe.org/4lun PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4lun RCSB], [https://www.ebi.ac.uk/pdbsum/4lun PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4lun ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
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[[https://www.uniprot.org/uniprot/NMD2_YEAST NMD2_YEAST]] Involved in nonsense-mediated decay of mRNAs containing premature stop codons. It interacts, via its C-terminus, with NAM7/UPF1. Could be involved in determining the efficiency of translational termination or reinitiation or factors involved in the initial assembly of an initiation- and termination-competent mRNP.
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[https://www.uniprot.org/uniprot/NMD2_YEAST NMD2_YEAST] Involved in nonsense-mediated decay of mRNAs containing premature stop codons. It interacts, via its C-terminus, with NAM7/UPF1. Could be involved in determining the efficiency of translational termination or reinitiation or factors involved in the initial assembly of an initiation- and termination-competent mRNP.
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Upf1, Upf2, and Upf3 are the principal regulators of nonsense-mediated mRNA decay (NMD), a cytoplasmic surveillance pathway that accelerates the degradation of mRNAs undergoing premature translation termination. These three proteins interact with each other, the ribosome, the translation termination machinery, and multiple mRNA decay factors, but the precise mechanism allowing the selective detection and degradation of nonsense-containing transcripts remains elusive. Here, we have determined the crystal structure of the N-terminal mIF4G domain from Saccharomyces cerevisiae Upf2 and identified a highly conserved region in this domain that is essential for NMD and independent of Upf2's binding sites for Upf1 and Upf3. Mutations within this conserved region not only inactivate NMD but also disrupt Upf2 binding to specific proteins, including Dbp6, a DEAD-box helicase. Although current models indicate that Upf2 functions principally as an activator of Upf1 and a bridge between Upf1 and Upf3, our data suggest that it may also serve as a platform for the association of additional factors that play roles in premature translation termination and NMD.
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A highly conserved region essential for NMD in the Upf2 N-terminal domain.,Fourati Z, Roy B, Millan C, Coureux PD, Kervestin S, van Tilbeurgh H, He F, Uson I, Jacobson A, Graille M J Mol Biol. 2014 Nov 11;426(22):3689-3702. doi: 10.1016/j.jmb.2014.09.015. Epub, 2014 Sep 30. PMID:25277656<ref>PMID:25277656</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 4lun" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Baker's yeast]]
 
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Courreux, P D]]
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[[Category: Saccharomyces cerevisiae S288C]]
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[[Category: Fourati, Z]]
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[[Category: Courreux PD]]
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[[Category: Graille, M]]
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[[Category: Fourati Z]]
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[[Category: He, F]]
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[[Category: Graille M]]
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[[Category: Jacobson, A]]
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[[Category: He F]]
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[[Category: Kervestin, S]]
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[[Category: Jacobson A]]
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[[Category: Millan, C]]
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[[Category: Kervestin S]]
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[[Category: Roy, B]]
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[[Category: Millan C]]
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[[Category: Tilbeurgh, H van]]
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[[Category: Roy B]]
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[[Category: Uson, I]]
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[[Category: Uson I]]
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[[Category: Heat-repeat]]
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[[Category: Van Tilbeurgh H]]
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[[Category: Mrna decay]]
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[[Category: Rna binding protein]]
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Current revision

Structure of the N-terminal mIF4G domain from S. cerevisiae Upf2, a protein involved in the degradation of mRNAs containing premature stop codons

PDB ID 4lun

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Proteopedia Page Contributors and Editors (what is this?)

OCA

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