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6u9e

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Current revision (09:29, 20 March 2024) (edit) (undo)
 
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<SX load='6u9e' size='340' side='right' viewer='molstar' caption='[[6u9e]], [[Resolution|resolution]] 4.21&Aring;' scene=''>
<SX load='6u9e' size='340' side='right' viewer='molstar' caption='[[6u9e]], [[Resolution|resolution]] 4.21&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[6u9e]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/"francisella_tularensis_subsp._novicida"_(larson_et_al._1955)_sjostedt_2005 "francisella tularensis subsp. novicida" (larson et al. 1955) sjostedt 2005]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6U9E OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6U9E FirstGlance]. <br>
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<table><tr><td colspan='2'>[[6u9e]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Francisella_tularensis_subsp._novicida Francisella tularensis subsp. novicida]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6U9E OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6U9E FirstGlance]. <br>
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</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">pdpA ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=264 "Francisella tularensis subsp. novicida" (Larson et al. 1955) Sjostedt 2005]), B4919_03525 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=264 "Francisella tularensis subsp. novicida" (Larson et al. 1955) Sjostedt 2005])</td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 4.21&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6u9e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6u9e OCA], [https://pdbe.org/6u9e PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6u9e RCSB], [https://www.ebi.ac.uk/pdbsum/6u9e PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6u9e ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6u9e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6u9e OCA], [https://pdbe.org/6u9e PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6u9e RCSB], [https://www.ebi.ac.uk/pdbsum/6u9e PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6u9e ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/Q7X3I9_FRANO Q7X3I9_FRANO]
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Francisella bacteria rely on a phylogenetically distinct type VI secretion system (T6SS) to escape host phagosomes and cause the fatal disease tularemia, but the structural and molecular mechanisms involved are unknown. Here we report the atomic structure of the Francisella T6SS central spike complex, obtained by cryo-electron microscopy. Our structural and functional studies demonstrate that, unlike the single-protein spike composition of other T6SS subtypes, Francisella T6SS's central spike is formed by two proteins, PdpA and VgrG, akin to T4-bacteriophage gp27 and gp5, respectively, and that PdpA has unique characteristics, including a putative cargo within its cavity and an N-terminal helical lid. Structure-guided mutagenesis demonstrates that the PdpA N-terminal lid and C-terminal spike are essential to Francisella T6SS function. PdpA is thus both an adaptor, connecting VgrG to the tube, and a likely carrier of secreted cargo. These findings are important to understanding Francisella pathogenicity and designing therapeutics to combat tularemia.
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Atomic Structure of the Francisella T6SS Central Spike Reveals a Unique alpha-Helical Lid and a Putative Cargo.,Yang X, Clemens DL, Lee BY, Cui Y, Zhou ZH, Horwitz MA Structure. 2019 Dec 3;27(12):1811-1819.e6. doi: 10.1016/j.str.2019.10.007. Epub, 2019 Oct 31. PMID:31677891<ref>PMID:31677891</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 6u9e" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</SX>
</SX>
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[[Category: Francisella tularensis subsp. novicida]]
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Clemens, D L]]
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[[Category: Clemens DL]]
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[[Category: Cui, Y]]
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[[Category: Cui Y]]
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[[Category: Horwitz, M A]]
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[[Category: Horwitz MA]]
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[[Category: Lee, B Y]]
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[[Category: Lee B-Y]]
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[[Category: Yang, X]]
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[[Category: Yang X]]
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[[Category: Zhou, Z H]]
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[[Category: Zhou ZH]]
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[[Category: Complex]]
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[[Category: T6ss central spike]]
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[[Category: Transport protein]]
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Current revision

Structure of PdpA-VgrG Complex, Lidless

6u9e, resolution 4.21Å

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