1ed7

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[[Image:1ed7.gif|left|200px]]
 
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==SOLUTION STRUCTURE OF THE CHITIN-BINDING DOMAIN OF BACILLUS CIRCULANS WL-12 CHITINASE A1==
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The line below this paragraph, containing "STRUCTURE_1ed7", creates the "Structure Box" on the page.
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<StructureSection load='1ed7' size='340' side='right'caption='[[1ed7]]' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1ed7]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Niallia_circulans Niallia circulans]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ED7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ED7 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ed7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ed7 OCA], [https://pdbe.org/1ed7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ed7 RCSB], [https://www.ebi.ac.uk/pdbsum/1ed7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ed7 ProSAT]</span></td></tr>
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{{STRUCTURE_1ed7| PDB=1ed7 | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CHIA1_NIACI CHIA1_NIACI]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ed/1ed7_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ed7 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The three-dimensional structure of the chitin-binding domain (ChBD) of chitinase A1 (ChiA1) from a Gram-positive bacterium, Bacillus circulans WL-12, was determined by means of multidimensional heteronuclear NMR methods. ChiA1 is a glycosidase that hydrolyzes chitin and is composed of an N-terminal catalytic domain, two fibronectin type III-like domains, and C-terminal ChBD(ChiA1) (45 residues, Ala(655)-Gln(699)), which binds specifically to insoluble chitin. ChBD(ChiA1) has a compact and globular structure with the topology of a twisted beta-sandwich. This domain contains two antiparallel beta-sheets, one composed of three strands and the other of two strands. The core region formed by the hydrophobic and aromatic residues makes the overall structure rigid and compact. The overall topology of ChBD(ChiA1) is similar to that of the cellulose-binding domain (CBD) of Erwinia chrysanthemi endoglucanase Z (CBD(EGZ)). However, ChBD(ChiA1) lacks the three aromatic residues aligned linearly and exposed to the solvent, which probably interact with cellulose in CBDs. Therefore, the binding mechanism of a group of ChBDs including ChBD(ChiA1) may be different from that proposed for CBDs.
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'''SOLUTION STRUCTURE OF THE CHITIN-BINDING DOMAIN OF BACILLUS CIRCULANS WL-12 CHITINASE A1'''
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Solution structure of the chitin-binding domain of Bacillus circulans WL-12 chitinase A1.,Ikegami T, Okada T, Hashimoto M, Seino S, Watanabe T, Shirakawa M J Biol Chem. 2000 May 5;275(18):13654-61. PMID:10788483<ref>PMID:10788483</ref>
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==Overview==
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The three-dimensional structure of the chitin-binding domain (ChBD) of chitinase A1 (ChiA1) from a Gram-positive bacterium, Bacillus circulans WL-12, was determined by means of multidimensional heteronuclear NMR methods. ChiA1 is a glycosidase that hydrolyzes chitin and is composed of an N-terminal catalytic domain, two fibronectin type III-like domains, and C-terminal ChBD(ChiA1) (45 residues, Ala(655)-Gln(699)), which binds specifically to insoluble chitin. ChBD(ChiA1) has a compact and globular structure with the topology of a twisted beta-sandwich. This domain contains two antiparallel beta-sheets, one composed of three strands and the other of two strands. The core region formed by the hydrophobic and aromatic residues makes the overall structure rigid and compact. The overall topology of ChBD(ChiA1) is similar to that of the cellulose-binding domain (CBD) of Erwinia chrysanthemi endoglucanase Z (CBD(EGZ)). However, ChBD(ChiA1) lacks the three aromatic residues aligned linearly and exposed to the solvent, which probably interact with cellulose in CBDs. Therefore, the binding mechanism of a group of ChBDs including ChBD(ChiA1) may be different from that proposed for CBDs.
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==About this Structure==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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1ED7 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_circulans Bacillus circulans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ED7 OCA].
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</div>
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<div class="pdbe-citations 1ed7" style="background-color:#fffaf0;"></div>
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==Reference==
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==See Also==
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Solution structure of the chitin-binding domain of Bacillus circulans WL-12 chitinase A1., Ikegami T, Okada T, Hashimoto M, Seino S, Watanabe T, Shirakawa M, J Biol Chem. 2000 May 5;275(18):13654-61. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10788483 10788483]
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*[[Chitinase 3D structures|Chitinase 3D structures]]
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[[Category: Bacillus circulans]]
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== References ==
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[[Category: Chitinase]]
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<references/>
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[[Category: Single protein]]
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__TOC__
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[[Category: Hashimoto, M.]]
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</StructureSection>
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[[Category: Ikegami, T.]]
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[[Category: Large Structures]]
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[[Category: Okada, T.]]
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[[Category: Niallia circulans]]
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[[Category: Seino, S.]]
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[[Category: Hashimoto M]]
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[[Category: Shirakawa, M.]]
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[[Category: Ikegami T]]
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[[Category: Watanabe, T.]]
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[[Category: Okada T]]
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[[Category: Twisted beta-sandwich]]
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[[Category: Seino S]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 14:57:27 2008''
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[[Category: Shirakawa M]]
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[[Category: Watanabe T]]

Current revision

SOLUTION STRUCTURE OF THE CHITIN-BINDING DOMAIN OF BACILLUS CIRCULANS WL-12 CHITINASE A1

PDB ID 1ed7

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