7of4
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 7of4 is ON HOLD Authors: Hillen, H.S., Lavdovskaia, E., Nadler, F., Hanitsch, E., Linden, A., Bohnsack, K.E., Urlaub, H., Richter-Dennerlein, R. De...) |
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| - | '''Unreleased structure''' | ||
| - | + | ==Structure of mature human mitochondrial ribosome large subunit in complex with GTPBP6 (PTC conformation 1).== | |
| + | <StructureSection load='7of4' size='340' side='right'caption='[[7of4]], [[Resolution|resolution]] 2.70Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[7of4]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7OF4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7OF4 FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.7Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=OMG:O2-METHYLGUANOSINE-5-MONOPHOSPHATE'>OMG</scene>, <scene name='pdbligand=OMU:O2-METHYLURIDINE+5-MONOPHOSPHATE'>OMU</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7of4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7of4 OCA], [https://pdbe.org/7of4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7of4 RCSB], [https://www.ebi.ac.uk/pdbsum/7of4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7of4 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/RM32_HUMAN RM32_HUMAN] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Ribosome biogenesis requires auxiliary factors to promote folding and assembly of ribosomal proteins and RNA. Particularly, maturation of the peptidyl transferase center (PTC) is mediated by conserved GTPases, but the molecular basis is poorly understood. Here, we define the mechanism of GTPase-driven maturation of the human mitochondrial large ribosomal subunit (mtLSU) using endogenous complex purification, in vitro reconstitution and cryo-EM. Structures of transient native mtLSU assembly intermediates that accumulate in GTPBP6-deficient cells reveal how the biogenesis factors GTPBP5, MTERF4 and NSUN4 facilitate PTC folding. Addition of recombinant GTPBP6 reconstitutes late mtLSU biogenesis in vitro and shows that GTPBP6 triggers a molecular switch and progression to a near-mature PTC state. Additionally, cryo-EM analysis of GTPBP6-treated mature mitochondrial ribosomes reveals the structural basis for the dual-role of GTPBP6 in ribosome biogenesis and recycling. Together, these results provide a framework for understanding step-wise PTC folding as a critical conserved quality control checkpoint. | ||
| - | + | Structural basis of GTPase-mediated mitochondrial ribosome biogenesis and recycling.,Hillen HS, Lavdovskaia E, Nadler F, Hanitsch E, Linden A, Bohnsack KE, Urlaub H, Richter-Dennerlein R Nat Commun. 2021 Jun 16;12(1):3672. doi: 10.1038/s41467-021-23702-y. PMID:34135319<ref>PMID:34135319</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 7of4" style="background-color:#fffaf0;"></div> |
| - | [[Category: | + | |
| - | [[Category: | + | ==See Also== |
| - | [[Category: | + | *[[GTP-binding protein 3D structures|GTP-binding protein 3D structures]] |
| - | [[Category: | + | *[[Ribosome 3D structures|Ribosome 3D structures]] |
| - | [[Category: | + | == References == |
| - | [[Category: | + | <references/> |
| - | [[Category: Urlaub | + | __TOC__ |
| + | </StructureSection> | ||
| + | [[Category: Homo sapiens]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Bohnsack KE]] | ||
| + | [[Category: Hanitsch E]] | ||
| + | [[Category: Hillen HS]] | ||
| + | [[Category: Lavdovskaia E]] | ||
| + | [[Category: Linden A]] | ||
| + | [[Category: Nadler F]] | ||
| + | [[Category: Richter-Dennerlein R]] | ||
| + | [[Category: Urlaub H]] | ||
Current revision
Structure of mature human mitochondrial ribosome large subunit in complex with GTPBP6 (PTC conformation 1).
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