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1eek

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[[Image:1eek.gif|left|200px]]
 
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==SOLUTION STRUCTURE OF A NONPOLAR, NON HYDROGEN BONDED BASE PAIR SURROGATE IN DNA.==
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The line below this paragraph, containing "STRUCTURE_1eek", creates the "Structure Box" on the page.
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<StructureSection load='1eek' size='340' side='right'caption='[[1eek]]' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1eek]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EEK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1EEK FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DFT:1-[2-DEOXYRIBOFURANOSYL]-2,4-DIFLUORO-5-METHYL-BENZENE-5MONOPHOSPHATE'>DFT</scene>, <scene name='pdbligand=MBZ:1-[2-DEOXYRIBOFURANOSYL]-4-METHYL-BENZOIMIDAZOLE-5-MONOPHOSPHATE'>MBZ</scene></td></tr>
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{{STRUCTURE_1eek| PDB=1eek | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1eek FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1eek OCA], [https://pdbe.org/1eek PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1eek RCSB], [https://www.ebi.ac.uk/pdbsum/1eek PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1eek ProSAT]</span></td></tr>
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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We describe the structure in aqueous solution of a DNA duplex containing a base pair that is structurally analogous to A-T but which lacks hydrogen bonds. Base analogues F (a nonpolar isostere of thymine) and Z (a nonpolar isostere of adenine) are paired opposite one another in a 12 base pair duplex. The sequence context is the binding site of recently studied transcription factor hSRY. The Z-F pair has been shown to be replicated surprisingly well and selectively by DNA polymerase enzymes, considering that it is destabilizing and lacks Watson-Crick hydrogen bonds. The enzymatic studies led to the suggestion that part of the functional activity arises because the pair resembles a natural one in geometry. The present results show that, despite the absence of Watson-Crick hydrogen bonds, the Z-F pair structurally resembles an A-T pair in the same context. This lends support to the proposal that shape matching is an important component in replication, and suggests the general utility of using Z-F as a nonpolar replacement for A-T in probing protein-DNA interactions.
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'''SOLUTION STRUCTURE OF A NONPOLAR, NON HYDROGEN BONDED BASE PAIR SURROGATE IN DNA.'''
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Solution Structure of a Nonpolar, Non-Hydrogen-Bonded Base Pair Surrogate in DNA.,Guckian KM, Krugh TR, Kool ET J Am Chem Soc. 2000 Jul 26;122(29):6841-6847. doi: 10.1021/ja994164v. PMID:20882115<ref>PMID:20882115</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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==About this Structure==
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</div>
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Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EEK OCA].
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<div class="pdbe-citations 1eek" style="background-color:#fffaf0;"></div>
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[[Category: Guckian, K M.]]
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== References ==
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[[Category: Kool, E T.]]
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<references/>
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[[Category: Krugh, T R.]]
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__TOC__
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[[Category: Dna]]
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</StructureSection>
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[[Category: Dna base analog]]
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[[Category: Large Structures]]
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[[Category: Isosteric]]
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[[Category: Guckian KM]]
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[[Category: Nonpolar]]
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[[Category: Kool ET]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 14:59:55 2008''
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[[Category: Krugh TR]]

Current revision

SOLUTION STRUCTURE OF A NONPOLAR, NON HYDROGEN BONDED BASE PAIR SURROGATE IN DNA.

PDB ID 1eek

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