We apologize for Proteopedia being slow to respond. For the past two years, a new implementation of Proteopedia has been being built. Soon, it will replace this 18-year old system. All existing content will be moved to the new system at a date that will be announced here.

2lel

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (06:55, 1 May 2024) (edit) (undo)
 
(One intermediate revision not shown.)
Line 1: Line 1:
==Structure of Cu(I)Cu(II)-CopK from Cupriavidus metallidurans CH34==
==Structure of Cu(I)Cu(II)-CopK from Cupriavidus metallidurans CH34==
-
<StructureSection load='2lel' size='340' side='right'caption='[[2lel]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
+
<StructureSection load='2lel' size='340' side='right'caption='[[2lel]]' scene=''>
== Structural highlights ==
== Structural highlights ==
-
<table><tr><td colspan='2'>[[2lel]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Cupmc Cupmc]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LEL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2LEL FirstGlance]. <br>
+
<table><tr><td colspan='2'>[[2lel]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Cupriavidus_metallidurans_CH34 Cupriavidus metallidurans CH34]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LEL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2LEL FirstGlance]. <br>
-
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=CU1:COPPER+(I)+ION'>CU1</scene></td></tr>
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
-
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">copK, Rmet_6108 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=266264 CUPMC])</td></tr>
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=CU1:COPPER+(I)+ION'>CU1</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2lel FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lel OCA], [https://pdbe.org/2lel PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2lel RCSB], [https://www.ebi.ac.uk/pdbsum/2lel PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2lel ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2lel FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lel OCA], [https://pdbe.org/2lel PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2lel RCSB], [https://www.ebi.ac.uk/pdbsum/2lel PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2lel ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
-
[[https://www.uniprot.org/uniprot/COPK_RALME COPK_RALME]] Involved in resistance to copper. Can bind up to 2 copper ions. Has higher affinity for Cu(+) than for Cu(2+).<ref>PMID:18533181</ref>
+
[https://www.uniprot.org/uniprot/COPK_CUPMC COPK_CUPMC] Involved in resistance to copper. Can bind up to 2 copper ions. Has higher affinity for Cu(+) than for Cu(2+).<ref>PMID:18533181</ref>
-
<div style="background-color:#fffaf0;">
+
-
== Publication Abstract from PubMed ==
+
-
The bacterium Cupriavidus metallidurans CH34 is resistant to high environmental concentrations of many metal ions. Upon copper challenge, it upregulates the periplasmic protein CopK (8.3 kDa). The function of CopK in the copper resistance response is ill-defined, but CopK demonstrates an intriguing cooperativity: occupation of a high-affinity Cu(I) binding site generates a high-affinity Cu(II) binding site, and the high-affinity Cu(II) binding enhances Cu(I) binding. Native CopK and targeted variants were examined by chromatographic, spectroscopic, and X-ray crystallographic probes. Structures of two distinct forms of Cu(I)Cu(II)-CopK were defined, and structural changes associated with occupation of the Cu(II) site were demonstrated. In solution, monomeric Cu(I)Cu(II)-CopK features the previously elucidated Cu(I) site in Cu(I)-CopK, formed from four S(delta) atoms of Met28, -38, -44, and -54 (site 4S). Binding of Cu(I) to apo-CopK induces a conformational change that releases the C-terminal beta-strand from the beta-sandwich structure. In turn, this allows His70 and N-terminal residues to form a large loop that includes the Cu(II) binding site. In crystals, a polymeric form of Cu(I)Cu(II)-CopK displays a Cu(I) site defined by the S(delta) atoms of Met26, -38, and -54 (site 3S) and an exogenous ligand (modeled as H(2)O) and a Cu(II) site that bridges dimeric CopK molecules. The 3S Cu(I) binding mode observed in crystals was demonstrated in solution in protein variant M44L where site 4S is disabled. The intriguing copper binding chemistry of CopK provides molecular insight into Cu(I) transfer processes. The adaptable nature of the Cu(I) coordination sphere in methionine-rich clusters allows copper to be relayed between clusters during transport across membranes in molecular pumps such as CusA and Ctr1.
+
-
 
+
-
Molecular basis of the cooperative binding of Cu(I) and Cu(II) to the CopK protein from Cupriavidus metallidurans CH34.,Ash MR, Chong LX, Maher MJ, Hinds MG, Xiao Z, Wedd AG Biochemistry. 2011 Nov 1;50(43):9237-47. Epub 2011 Oct 4. PMID:21936507<ref>PMID:21936507</ref>
+
-
 
+
-
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+
-
</div>
+
-
<div class="pdbe-citations 2lel" style="background-color:#fffaf0;"></div>
+
== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
-
[[Category: Cupmc]]
+
[[Category: Cupriavidus metallidurans CH34]]
[[Category: Large Structures]]
[[Category: Large Structures]]
-
[[Category: Chong, L X]]
+
[[Category: Chong LX]]
-
[[Category: Hinds, M G]]
+
[[Category: Hinds MG]]
-
[[Category: Wedd, A G]]
+
[[Category: Wedd AG]]
-
[[Category: Xiao, Z]]
+
[[Category: Xiao Z]]
-
[[Category: Binding cooperativity]]
+
-
[[Category: Copper protein]]
+
-
[[Category: Copper resistance]]
+
-
[[Category: Copper transport]]
+
-
[[Category: Metal binding protein]]
+

Current revision

Structure of Cu(I)Cu(II)-CopK from Cupriavidus metallidurans CH34

PDB ID 2lel

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2lel"
Personal tools