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2lj9

From Proteopedia

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Current revision

Partial 3d structure of the c-terminal part of the free arabidopsis thaliana cp12-2 in its oxidized form

Structural highlights

2lj9 is a 1 chain structure with sequence from Arabidopsis thaliana. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

CP122_ARATH Acts as a linker essential in the assembly of a core complex of PRK/GAPDH. Coordinates the reversible inactivation of chloroplast enzymes GAPDH and PRK during darkness in photosynthetic tissues.^[1] ^[2]

Publication Abstract from PubMed

Carbon assimilation in plants is regulated by the reduction of specific protein disulfides by light and their re-oxidation in the dark. The redox switch CP12 is an intrinsically disordered protein that can form two disulfide bridges. In the dark oxidized CP12 forms an inactive supramolecular complex with glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and phosphoribulokinase, two enzymes of the carbon assimilation cycle. Here we show that binding of CP12 to GAPDH, the first step of ternary complex formation, follows an integrated mechanism that combines conformational selection with induced folding steps. Initially, a CP12 conformation characterized by a circular structural motif including the C-terminal disulfide is selected by GAPDH. Subsequently, the induced folding of the flexible C-terminal tail of CP12 in the active site of GAPDH stabilizes the binary complex. Formation of several hydrogen bonds compensates the entropic cost of CP12 fixation and terminates the interaction mechanism that contributes to carbon assimilation control.

Conformational Selection and Folding-upon-binding of Intrinsically Disordered Protein CP12 Regulate Photosynthetic Enzymes Assembly.,Fermani S, Trivelli X, Sparla F, Thumiger A, Calvaresi M, Marri L, Falini G, Zerbetto F, Trost P J Biol Chem. 2012 Jun 15;287(25):21372-83. Epub 2012 Apr 18. PMID:22514274^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Marri L, Trost P, Pupillo P, Sparla F. Reconstitution and properties of the recombinant glyceraldehyde-3-phosphate dehydrogenase/CP12/phosphoribulokinase supramolecular complex of Arabidopsis. Plant Physiol. 2005 Nov;139(3):1433-43. Epub 2005 Oct 28. PMID:16258009 doi:10.1104/pp.105.068445
↑ Marri L, Pesaresi A, Valerio C, Lamba D, Pupillo P, Trost P, Sparla F. In vitro characterization of Arabidopsis CP12 isoforms reveals common biochemical and molecular properties. J Plant Physiol. 2010 Aug 15;167(12):939-50. doi: 10.1016/j.jplph.2010.02.008. PMID:20399532 doi:http://dx.doi.org/10.1016/j.jplph.2010.02.008
↑ Fermani S, Trivelli X, Sparla F, Thumiger A, Calvaresi M, Marri L, Falini G, Zerbetto F, Trost P. Conformational Selection and Folding-upon-binding of Intrinsically Disordered Protein CP12 Regulate Photosynthetic Enzymes Assembly. J Biol Chem. 2012 Jun 15;287(25):21372-83. Epub 2012 Apr 18. PMID:22514274 doi:10.1074/jbc.M112.350355

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2lj9"

@@ Line 1: / Line 1: @@
 ==Partial 3d structure of the c-terminal part of the free arabidopsis thaliana cp12-2 in its oxidized form==
-<StructureSection load='2lj9' size='340' side='right'caption='[[2lj9]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
+<StructureSection load='2lj9' size='340' side='right'caption='[[2lj9]]' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2lj9]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Arath Arath]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LJ9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2LJ9 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2lj9]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LJ9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2LJ9 FirstGlance]. <br>
-</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">At3g62410, CP12-2, T12C14_110 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 ARATH])</td></tr>
+</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2lj9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lj9 OCA], [https://pdbe.org/2lj9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2lj9 RCSB], [https://www.ebi.ac.uk/pdbsum/2lj9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2lj9 ProSAT]</span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2lj9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lj9 OCA], [https://pdbe.org/2lj9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2lj9 RCSB], [https://www.ebi.ac.uk/pdbsum/2lj9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2lj9 ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[https://www.uniprot.org/uniprot/CP122_ARATH CP122_ARATH]] Acts as a linker essential in the assembly of a core complex of PRK/GAPDH. Coordinates the reversible inactivation of chloroplast enzymes GAPDH and PRK during darkness in photosynthetic tissues.<ref>PMID:16258009</ref> <ref>PMID:20399532</ref>
+[https://www.uniprot.org/uniprot/CP122_ARATH CP122_ARATH] Acts as a linker essential in the assembly of a core complex of PRK/GAPDH. Coordinates the reversible inactivation of chloroplast enzymes GAPDH and PRK during darkness in photosynthetic tissues.<ref>PMID:16258009</ref> <ref>PMID:20399532</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 22: / Line 21: @@
 __TOC__
 </StructureSection>
-[[Category: Arath]]
+[[Category: Arabidopsis thaliana]]
 [[Category: Large Structures]]
-[[Category: Marri, L]]
+[[Category: Marri L]]
-[[Category: Sparla, F]]
+[[Category: Sparla F]]
-[[Category: Trivelli, X]]
+[[Category: Trivelli X]]
-[[Category: Trost, P]]
+[[Category: Trost P]]
-[[Category: Helix]]
-[[Category: Intrinsically disordered protein]]
-[[Category: Protein binding]]

2lj9

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Current revision

Partial 3d structure of the c-terminal part of the free arabidopsis thaliana cp12-2 in its oxidized form

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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