1eg0

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[[Image:1eg0.gif|left|200px]]
 
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==FITTING OF COMPONENTS WITH KNOWN STRUCTURE INTO AN 11.5 A CRYO-EM MAP OF THE E.COLI 70S RIBOSOME==
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The line below this paragraph, containing "STRUCTURE_1eg0", creates the "Structure Box" on the page.
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<SX load='1eg0' size='340' side='right' viewer='molstar' caption='[[1eg0]], [[Resolution|resolution]] 11.50&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1eg0]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EG0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1EG0 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 11.5&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=4SU:4-THIOURIDINE-5-MONOPHOSPHATE'>4SU</scene>, <scene name='pdbligand=5MU:5-METHYLURIDINE+5-MONOPHOSPHATE'>5MU</scene>, <scene name='pdbligand=H2U:5,6-DIHYDROURIDINE-5-MONOPHOSPHATE'>H2U</scene>, <scene name='pdbligand=N:ANY+5-MONOPHOSPHATE+NUCLEOTIDE'>N</scene>, <scene name='pdbligand=OMC:O2-METHYLYCYTIDINE-5-MONOPHOSPHATE'>OMC</scene>, <scene name='pdbligand=PSU:PSEUDOURIDINE-5-MONOPHOSPHATE'>PSU</scene></td></tr>
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{{STRUCTURE_1eg0| PDB=1eg0 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1eg0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1eg0 OCA], [https://pdbe.org/1eg0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1eg0 RCSB], [https://www.ebi.ac.uk/pdbsum/1eg0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1eg0 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RS4_BACSU RS4_BACSU] One of the primary rRNA binding proteins, it binds directly to 16S rRNA where it nucleates assembly of the body of the 30S subunit. With S5 and S12 plays an important role in translational accuracy; many suppressors of streptomycin-dependent mutants of protein S12 are found in this protein, some but not all of which decrease translational accuracy (ram, ribosomal ambiguity mutations). S4 represses its own expression; it is not know if this is at the level of translation or of mRNA stability.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/eg/1eg0_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1eg0 ConSurf].
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<div style="clear:both"></div>
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'''FITTING OF COMPONENTS WITH KNOWN STRUCTURE INTO AN 11.5 A CRYO-EM MAP OF THE E.COLI 70S RIBOSOME'''
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==See Also==
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*[[Ribosomal protein L11 3D structures|Ribosomal protein L11 3D structures]]
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*[[Ribosomal protein L6|Ribosomal protein L6]]
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==Overview==
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__TOC__
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Over 73,000 projections of the E. coli ribosome bound with formyl-methionyl initiator tRNAf(Met) were used to obtain an 11.5 A cryo-electron microscopy map of the complex. This map allows identification of RNA helices, peripheral proteins, and intersubunit bridges. Comparison of double-stranded RNA regions and positions of proteins identified in both cryo-EM and X-ray maps indicates good overall agreement but points to rearrangements of ribosomal components required for the subunit association. Fitting of known components of the 50S stalk base region into the map defines the architecture of the GTPase-associated center and reveals a major change in the orientation of the alpha-sarcin-ricin loop. Analysis of the bridging connections between the subunits provides insight into the dynamic signaling mechanism between the ribosomal subunits.
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</SX>
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==About this Structure==
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1EG0 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EG0 OCA].
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==Reference==
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Solution structure of the E. coli 70S ribosome at 11.5 A resolution., Gabashvili IS, Agrawal RK, Spahn CM, Grassucci RA, Svergun DI, Frank J, Penczek P, Cell. 2000 Mar 3;100(5):537-49. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10721991 10721991]
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[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Protein complex]]
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[[Category: Large Structures]]
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[[Category: Agrawal, R K.]]
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[[Category: Agrawal RK]]
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[[Category: Frank, J.]]
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[[Category: Frank J]]
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[[Category: Gabashvili, I S.]]
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[[Category: Gabashvili IS]]
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[[Category: Grassucci, R A.]]
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[[Category: Grassucci RA]]
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[[Category: Penczek, P.]]
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[[Category: Penczek P]]
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[[Category: Spahn, C M.T.]]
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[[Category: Spahn CMT]]
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[[Category: Svergun, D I.]]
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[[Category: Svergun DI]]
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[[Category: 70s ribosome]]
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[[Category: Low resolution model]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 15:03:07 2008''
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Current revision

FITTING OF COMPONENTS WITH KNOWN STRUCTURE INTO AN 11.5 A CRYO-EM MAP OF THE E.COLI 70S RIBOSOME

1eg0, resolution 11.50Å

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OCA

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