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| | ==NMR structure of OmpX in DPC micelles== | | ==NMR structure of OmpX in DPC micelles== |
| - | <StructureSection load='2m07' size='340' side='right'caption='[[2m07]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | + | <StructureSection load='2m07' size='340' side='right'caption='[[2m07]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2m07]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Ecoli Ecoli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2M07 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2M07 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2m07]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2M07 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2M07 FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1q9f|1q9f]], [[1qj8|1qj8]], [[2m06|2m06]]</div></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ompX, ybiG, b0814, JW0799 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>
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| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2m07 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2m07 OCA], [https://pdbe.org/2m07 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2m07 RCSB], [https://www.ebi.ac.uk/pdbsum/2m07 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2m07 ProSAT]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2m07 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2m07 OCA], [https://pdbe.org/2m07 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2m07 RCSB], [https://www.ebi.ac.uk/pdbsum/2m07 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2m07 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/OMPX_ECOLI OMPX_ECOLI] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Ecoli]] | + | [[Category: Escherichia coli K-12]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Etzkorn, M]] | + | [[Category: Etzkorn M]] |
| - | [[Category: Hagn, F X]] | + | [[Category: Hagn FX]] |
| - | [[Category: MPSbyNMR, Membrane Protein Structures by Solution NMR]]
| + | [[Category: Raschle T]] |
| - | [[Category: Raschle, T]] | + | [[Category: Wagner G]] |
| - | [[Category: Wagner, G]] | + | |
| - | [[Category: Beta barrel]]
| + | |
| - | [[Category: Membrane protein]]
| + | |
| - | [[Category: Membrane protein structures by solution nmr]]
| + | |
| - | [[Category: Mpsbynmr]]
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| - | [[Category: Psi-biology]]
| + | |
| - | [[Category: Structural genomic]]
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| Structural highlights
Function
OMPX_ECOLI
Publication Abstract from PubMed
Structural studies of membrane proteins are still hampered by difficulties of finding appropriate membrane mimicking media that maintain protein structure and function. Phospholipid nanodiscs seem promising to overcome the intrinsic problems of detergent containing environments. While nanodiscs can offer a near native environment, the large particle size complicates their routine use in the structural analysis of membrane proteins by solution NMR. Here, we introduce nanodiscs assembled from shorter ApoA-I protein variants that are of markedly smaller diameter and show that the resulting discs provide critical improvements for the structure determination of membrane proteins by NMR. Using the bacterial outer membrane protein OmpX as an example, we demonstrate that the combination of small nanodisc size, high deuteration levels of protein and lipids and the use of advanced non-uniform NMR sampling methods enable the NMR resonance assignment as well as the high-resolution structure determination of polytopic membrane proteins in a detergent-free, near-native lipid bilayer setting. By applying this method to bacteriorhodopsin we show that our smaller nanodiscs can also be beneficial for the structural characteri-zation of the important class of seven-transmembrane helical proteins. Our set of engineered nanodiscs of subsequently smaller diameters can be used to screen for optimal NMR spectral quality for any given membrane protein while still providing a functional environment. In addi-tion to their key improvements for de novo structure determination, due to their smaller size these nanodiscs enable the investigation of inter-actions between membrane proteins and their (soluble) partner proteins, unbiased by the presence of detergents that might disrupt biological relevant interactions.
Optimized Phospholipid Bilayer Nanodiscs Facilitate High-Resolution Structure Determination of Membrane Proteins.,Hagn F, Etzkorn M, Raschle T, Wagner G J Am Chem Soc. 2013 Jan 8. PMID:23294159[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Hagn F, Etzkorn M, Raschle T, Wagner G. Optimized Phospholipid Bilayer Nanodiscs Facilitate High-Resolution Structure Determination of Membrane Proteins. J Am Chem Soc. 2013 Jan 8. PMID:23294159 doi:http://dx.doi.org/10.1021/ja310901f
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