7eu9
From Proteopedia
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(New page: ==== <StructureSection load='7eu9' size='340' side='right'caption='7eu9' scene=''> == Structural highlights == <table><tr><td colspan='2'>Full crystallographic information is availabl...) |
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| - | ==== | + | ==Crystal structure of the selenomethionine(SeMet)-derived Cas12i1 R-loop complex before target DNA cleavage== |
| - | <StructureSection load='7eu9' size='340' side='right'caption='[[7eu9]]' scene=''> | + | <StructureSection load='7eu9' size='340' side='right'caption='[[7eu9]], [[Resolution|resolution]] 2.35Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7eu9]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Lachnospiraceae_bacterium_ND2006 Lachnospiraceae bacterium ND2006]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7EU9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7EU9 FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7eu9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7eu9 OCA], [https://pdbe.org/7eu9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7eu9 RCSB], [https://www.ebi.ac.uk/pdbsum/7eu9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7eu9 ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.35Å</td></tr> |
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7eu9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7eu9 OCA], [https://pdbe.org/7eu9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7eu9 RCSB], [https://www.ebi.ac.uk/pdbsum/7eu9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7eu9 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Cas12i is a newly identified member of the functionally diverse type V CRISPR-Cas effectors. Although Cas12i has the potential to serve as genome-editing tool, its structural and functional characteristics need to be investigated in more detail before effective application. Here we report the crystal structures of the Cas12i1 R-loop complexes before and after target DNA cleavage to elucidate the mechanisms underlying target DNA duplex unwinding, R-loop formation and cis cleavage. The structure of the R-loop complex after target DNA cleavage also provides information regarding trans cleavage. Besides, we report a crystal structure of the Cas12i1 binary complex interacting with a pseudo target oligonucleotide, which mimics target interrogation. Upon target DNA duplex binding, the Cas12i1 PAM-interacting cleft undergoes a remarkable open-to-closed adjustment. Notably, a zipper motif in the Helical-I domain facilitates unzipping of the target DNA duplex. Formation of the 19-bp crRNA-target DNA strand heteroduplex in the R-loop complexes triggers a conformational rearrangement and unleashes the DNase activity. This study provides valuable insights for developing Cas12i1 into a reliable genome-editing tool. | ||
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| + | Mechanistic insights into the R-loop formation and cleavage in CRISPR-Cas12i1.,Zhang B, Luo D, Li Y, Perculija V, Chen J, Lin J, Ye Y, Ouyang S Nat Commun. 2021 Jun 9;12(1):3476. doi: 10.1038/s41467-021-23876-5. PMID:34108490<ref>PMID:34108490</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7eu9" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Lachnospiraceae bacterium ND2006]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: | + | [[Category: Li Y]] |
| + | [[Category: Luo DY]] | ||
| + | [[Category: OuYang SY]] | ||
| + | [[Category: Zhang B]] | ||
Current revision
Crystal structure of the selenomethionine(SeMet)-derived Cas12i1 R-loop complex before target DNA cleavage
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