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| | ==HADDOCK model of MyT1 F4F5 - DNA complex== | | ==HADDOCK model of MyT1 F4F5 - DNA complex== |
| - | <StructureSection load='2mf8' size='340' side='right'caption='[[2mf8]], [[NMR_Ensembles_of_Models | 1 NMR models]]' scene=''> | + | <StructureSection load='2mf8' size='340' side='right'caption='[[2mf8]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2mf8]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MF8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2MF8 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2mf8]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MF8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2MF8 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2jyd|2jyd]]</div></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Myt1, Kiaa0835, Nzf2 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>
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| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2mf8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mf8 OCA], [https://pdbe.org/2mf8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2mf8 RCSB], [https://www.ebi.ac.uk/pdbsum/2mf8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2mf8 ProSAT]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2mf8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mf8 OCA], [https://pdbe.org/2mf8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2mf8 RCSB], [https://www.ebi.ac.uk/pdbsum/2mf8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2mf8 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[https://www.uniprot.org/uniprot/MYT1_MOUSE MYT1_MOUSE]] Binds to the promoter regions of proteolipid proteins of the central nervous system. May play a role in the development of neurons and oligodendrogalia in the CNS. May regulate a critical transition point in oligodendrocyte lineage development by modulating oligodendrocyte progenitor proliferation relative to terminal differentiation and up-regulation of myelin gene transcription (By similarity).<ref>PMID:9373037</ref>
| + | [https://www.uniprot.org/uniprot/MYT1_MOUSE MYT1_MOUSE] Binds to the promoter regions of proteolipid proteins of the central nervous system. May play a role in the development of neurons and oligodendrogalia in the CNS. May regulate a critical transition point in oligodendrocyte lineage development by modulating oligodendrocyte progenitor proliferation relative to terminal differentiation and up-regulation of myelin gene transcription (By similarity).<ref>PMID:9373037</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Lk3 transgenic mice]] | + | [[Category: Mus musculus]] |
| - | [[Category: Connell, M R.O]]
| + | [[Category: Cubeddu L]] |
| - | [[Category: Cubeddu, L]] | + | [[Category: Gamsjaeger R]] |
| - | [[Category: Gamsjaeger, R]] | + | [[Category: Kwan AH]] |
| - | [[Category: Kwan, A H]] | + | [[Category: Lowry JA]] |
| - | [[Category: Lowry, J A]] | + | [[Category: Mackay JP]] |
| - | [[Category: Mackay, J P]] | + | [[Category: Matthews JM]] |
| - | [[Category: Matthews, J M]] | + | [[Category: O'Connell MR]] |
| - | [[Category: Shepherd, N E]] | + | [[Category: Shepherd NE]] |
| - | [[Category: Swanton, M K]] | + | [[Category: Swanton MK]] |
| - | [[Category: Vandevenne, M]] | + | [[Category: Vandevenne M]] |
| - | [[Category: Metal binding protein-dna complex]] | + | |
| - | [[Category: Myt1]]
| + | |
| - | [[Category: Zinc finger]]
| + | |
| Structural highlights
Function
MYT1_MOUSE Binds to the promoter regions of proteolipid proteins of the central nervous system. May play a role in the development of neurons and oligodendrogalia in the CNS. May regulate a critical transition point in oligodendrocyte lineage development by modulating oligodendrocyte progenitor proliferation relative to terminal differentiation and up-regulation of myelin gene transcription (By similarity).[1]
Publication Abstract from PubMed
Myelin transcription factor 1 (MyT1/NZF-2), a member of the neural zinc-finger (NZF) protein family, is a transcription factor that plays a central role in the developing central nervous system. It has also recently been shown that, in combination with two other transcription factors, the highly similar paralog MyT1L is able to direct the differentiation of murine and human stem cells into functional neurons. MyT1 contains seven zinc fingers (ZnFs) that are highly conserved throughout the protein and throughout the NZF family. We recently presented a model for the interaction of the fifth ZnF of MyT1 with a DNA sequence derived from the promoter of the retinoic acid receptor (RARE) gene. Here, we have used NMR spectroscopy, in combination with surface plasmon resonance and data-driven molecular docking, to delineate the mechanism of DNA binding for double ZnF polypeptides derived from MyT1. Our data indicate that a two-ZnF unit interacts with the major groove of the entire RARE motif and that both fingers bind in an identical manner and with overall two-fold rotational symmetry, consistent with the palindromic nature of the target DNA. Several key residues located in one of the irregular loops of the ZnFs are utilized to achieve specific binding. Analysis of the human and mouse genomes based on our structural data reveals three putative MyT1 target genes involved in neuronal development.
A structural analysis of DNA binding by Myelin Transcription Factor 1 double zinc fingers.,Gamsjaeger R, O'Connell MR, Cubeddu L, Shepherd NE, Lowry JA, Kwan AH, Vandevenne M, Swanton MK, Matthews JM, Mackay JP J Biol Chem. 2013 Oct 6. PMID:24097990[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Kim JG, Armstrong RC, v Agoston D, Robinsky A, Wiese C, Nagle J, Hudson LD. Myelin transcription factor 1 (Myt1) of the oligodendrocyte lineage, along with a closely related CCHC zinc finger, is expressed in developing neurons in the mammalian central nervous system. J Neurosci Res. 1997 Oct 15;50(2):272-90. PMID:9373037
- ↑ Gamsjaeger R, O'Connell MR, Cubeddu L, Shepherd NE, Lowry JA, Kwan AH, Vandevenne M, Swanton MK, Matthews JM, Mackay JP. A structural analysis of DNA binding by Myelin Transcription Factor 1 double zinc fingers. J Biol Chem. 2013 Oct 6. PMID:24097990 doi:http://dx.doi.org/10.1074/jbc.M113.482075
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