7n1s
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 7n1s is ON HOLD Authors: Description: Category: Unreleased Structures) |
|||
(3 intermediate revisions not shown.) | |||
Line 1: | Line 1: | ||
- | '''Unreleased structure''' | ||
- | + | ==Crystal Structure Analysis of Xac Nucleotide Pyrophosphatase/Phosphodiesterase== | |
+ | <StructureSection load='7n1s' size='340' side='right'caption='[[7n1s]], [[Resolution|resolution]] 2.00Å' scene=''> | ||
+ | == Structural highlights == | ||
+ | <table><tr><td colspan='2'>[[7n1s]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Xanthomonas_citri Xanthomonas citri]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7N1S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7N1S FirstGlance]. <br> | ||
+ | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2Å</td></tr> | ||
+ | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | ||
+ | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7n1s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7n1s OCA], [https://pdbe.org/7n1s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7n1s RCSB], [https://www.ebi.ac.uk/pdbsum/7n1s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7n1s ProSAT]</span></td></tr> | ||
+ | </table> | ||
+ | == Function == | ||
+ | [https://www.uniprot.org/uniprot/A0A0U5FM15_XANCI A0A0U5FM15_XANCI] | ||
+ | <div style="background-color:#fffaf0;"> | ||
+ | == Publication Abstract from PubMed == | ||
+ | The metazoan innate immune second messenger 2'3'-cGAMP is present both inside and outside cells. However, only extracellular cGAMP can be negatively regulated by the extracellular hydrolase ENPP1. Here, we determine whether ENPP1's regulation of extracellular cGAMP is a ubiquitous mechanism of attenuating stimulator of interferon genes (STING) signaling. We identified ENPP1H362A, a point mutation that cannot degrade the 2'-5' linkage in cGAMP while maintaining otherwise normal function. The selectivity of this histidine is conserved down to bacterial nucleotide pyrophosphatase/phosphodiesterase (NPP), allowing structural analysis and suggesting an unexplored ancient history of 2'-5' cyclic dinucleotides. Enpp1H362A mice demonstrated that extracellular cGAMP is not responsible for the devastating phenotype in ENPP1-null humans and mice but is responsible for antiviral immunity and systemic inflammation. Our data define extracellular cGAMP as a pivotal STING activator, identify an evolutionarily critical role for ENPP1 in regulating inflammation, and suggest a therapeutic strategy for viral and inflammatory conditions by manipulating ENPP1 activity. | ||
- | + | ENPP1's regulation of extracellular cGAMP is a ubiquitous mechanism of attenuating STING signaling.,Carozza JA, Cordova AF, Brown JA, AlSaif Y, Bohnert V, Cao X, Mardjuki RE, Skariah G, Fernandez D, Li L Proc Natl Acad Sci U S A. 2022 May 24;119(21):e2119189119. doi: , 10.1073/pnas.2119189119. Epub 2022 May 19. PMID:35588451<ref>PMID:35588451</ref> | |
- | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
- | [[Category: | + | </div> |
+ | <div class="pdbe-citations 7n1s" style="background-color:#fffaf0;"></div> | ||
+ | == References == | ||
+ | <references/> | ||
+ | __TOC__ | ||
+ | </StructureSection> | ||
+ | [[Category: Large Structures]] | ||
+ | [[Category: Xanthomonas citri]] | ||
+ | [[Category: Brown JA]] | ||
+ | [[Category: Carozza JA]] | ||
+ | [[Category: Fernandez D]] | ||
+ | [[Category: Li L]] |
Current revision
Crystal Structure Analysis of Xac Nucleotide Pyrophosphatase/Phosphodiesterase
|