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1eiz

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[[Image:1eiz.jpg|left|200px]]
 
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==FTSJ RNA METHYLTRANSFERASE COMPLEXED WITH S-ADENOSYLMETHIONINE==
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The line below this paragraph, containing "STRUCTURE_1eiz", creates the "Structure Box" on the page.
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<StructureSection load='1eiz' size='340' side='right'caption='[[1eiz]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1eiz]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EIZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1EIZ FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene></td></tr>
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{{STRUCTURE_1eiz| PDB=1eiz | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1eiz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1eiz OCA], [https://pdbe.org/1eiz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1eiz RCSB], [https://www.ebi.ac.uk/pdbsum/1eiz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1eiz ProSAT]</span></td></tr>
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</table>
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'''FTSJ RNA METHYLTRANSFERASE COMPLEXED WITH S-ADENOSYLMETHIONINE'''
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== Function ==
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[https://www.uniprot.org/uniprot/RLME_ECOLI RLME_ECOLI] Specifically methylates the uridine in position 2552 of 23S rRNA at the 2'-O position of the ribose in the fully assembled 50S ribosomal subunit.<ref>PMID:10748051</ref>
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== Evolutionary Conservation ==
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==Overview==
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[[Image:Consurf_key_small.gif|200px|right]]
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Structural, biochemical, and genetic techniques were applied to investigate the function of FtsJ, a recently identified heat shock protein. FtsJ is well conserved, from bacteria to humans. The 1.5 A crystal structure of FtsJ in complex with its cofactor S-adenosylmethionine revealed that FtsJ has a methyltransferase fold. The molecular surface of FtsJ exposes a putative nucleic acid binding groove composed of highly conserved, positively charged residues. Substrate analysis showed that FtsJ methylates 23S rRNA within 50S ribosomal subunits in vitro and in vivo. Null mutations in ftsJ show a dramatically altered ribosome profile, a severe growth disadvantage, and a temperature-sensitive phenotype. Our results reveal an unexpected link between the heat shock response and RNA metabolism.
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Check<jmol>
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<jmolCheckbox>
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==About this Structure==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ei/1eiz_consurf.spt"</scriptWhenChecked>
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1EIZ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EIZ OCA].
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==Reference==
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</jmolCheckbox>
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RNA methylation under heat shock control., Bugl H, Fauman EB, Staker BL, Zheng F, Kushner SR, Saper MA, Bardwell JC, Jakob U, Mol Cell. 2000 Aug;6(2):349-60. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10983982 10983982]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1eiz ConSurf].
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<div style="clear:both"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Bardwell, J C.A.]]
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[[Category: Bardwell JCA]]
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[[Category: Bugl, H.]]
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[[Category: Bugl H]]
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[[Category: Fauman, E B.]]
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[[Category: Fauman EB]]
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[[Category: Jakob, U.]]
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[[Category: Jakob U]]
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[[Category: Kushner, S R.]]
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[[Category: Kushner SR]]
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[[Category: Saper, M A.]]
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[[Category: Saper MA]]
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[[Category: Staker, B L.]]
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[[Category: Staker BL]]
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[[Category: Zheng, F.]]
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[[Category: Zheng F]]
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[[Category: 23s ribosomal rna]]
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[[Category: Adenosyl methionine]]
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[[Category: Adomet]]
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[[Category: Ftsj]]
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[[Category: Heat shock protein]]
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[[Category: Methyltransferase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 15:09:25 2008''
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Current revision

FTSJ RNA METHYLTRANSFERASE COMPLEXED WITH S-ADENOSYLMETHIONINE

PDB ID 1eiz

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