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2mlg

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==Stf76 from the Sulfolobus islandicus plasmid-virus pSSVx==
==Stf76 from the Sulfolobus islandicus plasmid-virus pSSVx==
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<StructureSection load='2mlg' size='340' side='right'caption='[[2mlg]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
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<StructureSection load='2mlg' size='340' side='right'caption='[[2mlg]]' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[2mlg]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Fuselloviridae Fuselloviridae]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MLG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2MLG FirstGlance]. <br>
<table><tr><td colspan='2'>[[2mlg]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Fuselloviridae Fuselloviridae]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MLG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2MLG FirstGlance]. <br>
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</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2mlg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mlg OCA], [https://pdbe.org/2mlg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2mlg RCSB], [https://www.ebi.ac.uk/pdbsum/2mlg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2mlg ProSAT]</span></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2mlg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mlg OCA], [https://pdbe.org/2mlg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2mlg RCSB], [https://www.ebi.ac.uk/pdbsum/2mlg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2mlg ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/O05472_SULIS O05472_SULIS]
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The hybrid plasmid-virus pSSVx from Sulfolobus islandicus presents an open reading frame encoding a 76 amino acid protein, namely Stf76, that does not show significant sequence homology with any protein with known 3D structure. The recombinant protein recognizes specifically two DNA-binding sites located in its own promoter, thus suggesting an auto-regulated role of its expression. Circular dichroism, spectrofluorimetric, light scattering and isothermal titration calorimetry experiments indicated a 2:1 molar ratio (protein:DNA) upon binding to the DNA target containing a single site. Furthermore, the solution structure of Stf76, determined by nuclear magnetic resonance (NMR) using chemical shift Rosetta software, has shown that the protein assumes a winged helix-turn-helix fold. NMR chemical shift perturbation analysis has been performed for the identification of the residues responsible for DNA interaction. In addition, a model of the Stf76-DNA complex has been built using as template a structurally related homolog.
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Structural and functional studies of Stf76 from the Sulfolobus islandicus plasmid-virus pSSVx: a novel peculiar member of the winged helix-turn-helix transcription factor family.,Contursi P, Farina B, Pirone L, Fusco S, Russo L, Bartolucci S, Fattorusso R, Pedone E Nucleic Acids Res. 2014 May 1;42(9):5993-6011. doi: 10.1093/nar/gku215. Epub 2014, Mar 25. PMID:24682827<ref>PMID:24682827</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2mlg" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Fuselloviridae]]
[[Category: Fuselloviridae]]
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Farina, B]]
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[[Category: Farina B]]
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[[Category: Fattorusso, R]]
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[[Category: Fattorusso R]]
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[[Category: Russo, L]]
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[[Category: Russo L]]
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[[Category: Dna binding protein]]
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[[Category: Winged helix domain]]
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Current revision

Stf76 from the Sulfolobus islandicus plasmid-virus pSSVx

PDB ID 2mlg

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