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| | ==Solution structure of N terminal domain of the MuB AAA+ ATPase== | | ==Solution structure of N terminal domain of the MuB AAA+ ATPase== |
| - | <StructureSection load='2mqk' size='340' side='right'caption='[[2mqk]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | + | <StructureSection load='2mqk' size='340' side='right'caption='[[2mqk]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2mqk]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bpmu Bpmu]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MQK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2MQK FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2mqk]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_virus_Mu Escherichia virus Mu]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MQK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2MQK FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">B, Mup04 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10677 BPMU])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Adenosinetriphosphatase Adenosinetriphosphatase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.1.3 3.6.1.3] </span></td></tr>
| + | |
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2mqk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mqk OCA], [https://pdbe.org/2mqk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2mqk RCSB], [https://www.ebi.ac.uk/pdbsum/2mqk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2mqk ProSAT]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2mqk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mqk OCA], [https://pdbe.org/2mqk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2mqk RCSB], [https://www.ebi.ac.uk/pdbsum/2mqk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2mqk ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[https://www.uniprot.org/uniprot/TARGB_BPMU TARGB_BPMU]] Selects the target DNA sites for transposition. Recruits DDE-recombinase A to the target sites and catalytically activates it. Displays non-specific DNA-binding properties. Polymerizes as helical filaments around the DNA. Coating of the DNA by the target DNA activator B might play a role in favoring target-primed replication over integration. Prevents self-integration into an integrated copy of the viral genome. This mechanism is called target immunity and is achieved by two mechanisms: first, the target DNA activator B dissociates from the viral genome ends upon interaction in cis with DDE-recombinase A, which makes the viral genome ends a poor target for new insertions. Second, the interior of the viral genome may also ne protected from integration events by the target DNA activator B being strongly bound throughout the whole viral genome.<ref>PMID:11298282</ref> <ref>PMID:14661976</ref> <ref>PMID:1646076</ref> <ref>PMID:17709741</ref> <ref>PMID:17988683</ref> <ref>PMID:20226074</ref> <ref>PMID:23776210</ref> <ref>PMID:24478936</ref>
| + | [https://www.uniprot.org/uniprot/TARGB_BPMU TARGB_BPMU] Selects the target DNA sites for transposition. Recruits DDE-recombinase A to the target sites and catalytically activates it. Displays non-specific DNA-binding properties. Polymerizes as helical filaments around the DNA. Coating of the DNA by the target DNA activator B might play a role in favoring target-primed replication over integration. Prevents self-integration into an integrated copy of the viral genome. This mechanism is called target immunity and is achieved by two mechanisms: first, the target DNA activator B dissociates from the viral genome ends upon interaction in cis with DDE-recombinase A, which makes the viral genome ends a poor target for new insertions. Second, the interior of the viral genome may also ne protected from integration events by the target DNA activator B being strongly bound throughout the whole viral genome.<ref>PMID:11298282</ref> <ref>PMID:14661976</ref> <ref>PMID:1646076</ref> <ref>PMID:17709741</ref> <ref>PMID:17988683</ref> <ref>PMID:20226074</ref> <ref>PMID:23776210</ref> <ref>PMID:24478936</ref> |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Adenosinetriphosphatase]] | + | [[Category: Escherichia virus Mu]] |
| - | [[Category: Bpmu]]
| + | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Campos-Olivas, R]] | + | [[Category: Campos-Olivas R]] |
| - | [[Category: Dramicanin, M]] | + | [[Category: Dramicanin M]] |
| - | [[Category: Lopez-Mendez, B]] | + | [[Category: Lopez-Mendez B]] |
| - | [[Category: Ramon-Maiques, S]] | + | [[Category: Ramon-Maiques S]] |
| - | [[Category: Hydrolase]]
| + | |
| Structural highlights
Function
TARGB_BPMU Selects the target DNA sites for transposition. Recruits DDE-recombinase A to the target sites and catalytically activates it. Displays non-specific DNA-binding properties. Polymerizes as helical filaments around the DNA. Coating of the DNA by the target DNA activator B might play a role in favoring target-primed replication over integration. Prevents self-integration into an integrated copy of the viral genome. This mechanism is called target immunity and is achieved by two mechanisms: first, the target DNA activator B dissociates from the viral genome ends upon interaction in cis with DDE-recombinase A, which makes the viral genome ends a poor target for new insertions. Second, the interior of the viral genome may also ne protected from integration events by the target DNA activator B being strongly bound throughout the whole viral genome.[1] [2] [3] [4] [5] [6] [7] [8]
References
- ↑ Roldan LA, Baker TA. Differential role of the Mu B protein in phage Mu integration vs. replication: mechanistic insights into two transposition pathways. Mol Microbiol. 2001 Apr;40(1):141-55. PMID:11298282
- ↑ Goldhaber-Gordon I, Early MH, Baker TA. MuA transposase separates DNA sequence recognition from catalysis. Biochemistry. 2003 Dec 16;42(49):14633-42. PMID:14661976 doi:http://dx.doi.org/10.1021/bi035360o
- ↑ Baker TA, Mizuuchi M, Mizuuchi K. MuB protein allosterically activates strand transfer by the transposase of phage Mu. Cell. 1991 Jun 14;65(6):1003-13. PMID:1646076
- ↑ Tan X, Mizuuchi M, Mizuuchi K. DNA transposition target immunity and the determinants of the MuB distribution patterns on DNA. Proc Natl Acad Sci U S A. 2007 Aug 28;104(35):13925-9. Epub 2007 Aug 20. PMID:17709741 doi:http://dx.doi.org/10.1073/pnas.0706564104
- ↑ Lemberg KM, Schweidenback CT, Baker TA. The dynamic Mu transpososome: MuB activation prevents disintegration. J Mol Biol. 2007 Dec 14;374(5):1158-71. Epub 2007 Oct 3. PMID:17988683 doi:http://dx.doi.org/10.1016/j.jmb.2007.09.079
- ↑ Ge J, Lou Z, Harshey RM. Immunity of replicating Mu to self-integration: a novel mechanism employing MuB protein. Mob DNA. 2010 Feb 1;1(1):8. doi: 10.1186/1759-8753-1-8. PMID:20226074 doi:http://dx.doi.org/10.1186/1759-8753-1-8
- ↑ Mizuno N, Dramicanin M, Mizuuchi M, Adam J, Wang Y, Han YW, Yang W, Steven AC, Mizuuchi K, Ramon-Maiques S. MuB is an AAA+ ATPase that forms helical filaments to control target selection for DNA transposition. Proc Natl Acad Sci U S A. 2013 Jun 17. PMID:23776210 doi:10.1073/pnas.1309499110
- ↑ Dramicanin M, Ramon-Maiques S. MuB gives a new twist to target DNA selection. Mob Genet Elements. 2013 Sep 1;3(5):e27515. Epub 2013 Dec 12. PMID:24478936 doi:http://dx.doi.org/10.4161/mge.27515
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