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1eji

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[[Image:1eji.jpg|left|200px]]
 
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==RECOMBINANT SERINE HYDROXYMETHYLTRANSFERASE (MOUSE)==
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The line below this paragraph, containing "STRUCTURE_1eji", creates the "Structure Box" on the page.
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<StructureSection load='1eji' size='340' side='right'caption='[[1eji]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1eji]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EJI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1EJI FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.9&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=PLG:N-GLYCINE-[3-HYDROXY-2-METHYL-5-PHOSPHONOOXYMETHYL-PYRIDIN-4-YL-METHANE]'>PLG</scene>, <scene name='pdbligand=THF:5-HYDROXYMETHYLENE-6-HYDROFOLIC+ACID'>THF</scene></td></tr>
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{{STRUCTURE_1eji| PDB=1eji | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1eji FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1eji OCA], [https://pdbe.org/1eji PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1eji RCSB], [https://www.ebi.ac.uk/pdbsum/1eji PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1eji ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/GLYC_MOUSE GLYC_MOUSE] Interconversion of serine and glycine.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ej/1eji_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1eji ConSurf].
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<div style="clear:both"></div>
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'''RECOMBINANT SERINE HYDROXYMETHYLTRANSFERASE (MOUSE)'''
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==See Also==
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*[[Serine hydroxymethyltransferase 3D structures|Serine hydroxymethyltransferase 3D structures]]
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__TOC__
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==Overview==
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</StructureSection>
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Serine hydroxymethyltransferase (SHMT) is a pyridoxal phosphate-dependent enzyme that catalyzes the reversible conversion of serine and tetrahydrofolate to glycine and methylenetetrahydrofolate. This reaction generates single carbon units for purine, thymidine, and methionine biosynthesis. The enzyme is a homotetramer comprising two obligate dimers and four pyridoxal phosphate-bound active sites. The mammalian enzyme is present in cells in both catalytically active and inactive forms. The inactive form is a ternary complex that results from the binding of glycine and 5-formyltetrahydrofolate polyglutamate, a slow tight-binding inhibitor. The crystal structure of a close analogue of the inactive form of murine cytoplasmic SHMT (cSHMT), lacking only the polyglutamate tail of the inhibitor, has been determined to 2.9 A resolution. This first structure of a ligand-bound mammalian SHMT allows identification of amino acid residues involved in substrate binding and catalysis. It also reveals that the two obligate dimers making up a tetramer are not equivalent; one can be described as "tight-binding" and the other as "loose-binding" for folate. Both active sites of the tight-binding dimer are occupied by 5-formyltetrahydrofolate (5-formylTHF), whose N5-formyl carbon is within 4 A of the glycine alpha-carbon of the glycine-pyridoxal phosphate complex; the complex appears to be primarily in its quinonoid form. In the loose-binding dimer, 5-formylTHF is present in only one of the active sites, and its N5-formyl carbon is 5 A from the glycine alpha-carbon. The pyridoxal phosphates appear to be primarily present as geminal diamine complexes, with bonds to both glycine and the active site lysine. This structure suggests that only two of the four catalytic sites on SHMT are catalytically competent and that the cSHMT-glycine-5-formylTHF ternary complex is an intermediate state analogue of the catalytic complex associated with serine and glycine interconversion.
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[[Category: Large Structures]]
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==About this Structure==
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1EJI is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EJI OCA].
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==Reference==
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Structure of a murine cytoplasmic serine hydroxymethyltransferase quinonoid ternary complex: evidence for asymmetric obligate dimers., Szebenyi DM, Liu X, Kriksunov IA, Stover PJ, Thiel DJ, Biochemistry. 2000 Nov 7;39(44):13313-23. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11063567 11063567]
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[[Category: Glycine hydroxymethyltransferase]]
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[[Category: Mus musculus]]
[[Category: Mus musculus]]
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[[Category: Single protein]]
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[[Category: Kriksunov IA]]
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[[Category: Kriksunov, I A.]]
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[[Category: Liu X]]
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[[Category: Liu, X.]]
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[[Category: Stover PJ]]
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[[Category: Stover, P J.]]
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[[Category: Szebenyi DME]]
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[[Category: Szebenyi, D M.E.]]
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[[Category: Thiel DJ]]
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[[Category: Thiel, D J.]]
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[[Category: Asymmetric dimer]]
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[[Category: Pyridoxal 5'-phosphate]]
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[[Category: Serine-glycine conversion]]
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[[Category: Tetrahydrofolate]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 15:10:45 2008''
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Current revision

RECOMBINANT SERINE HYDROXYMETHYLTRANSFERASE (MOUSE)

PDB ID 1eji

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