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2n3l

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==Solution structure of RNA recognition motif-1 of Plasmodium falciparum serine/arginine-rich protein 1.==
==Solution structure of RNA recognition motif-1 of Plasmodium falciparum serine/arginine-rich protein 1.==
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<StructureSection load='2n3l' size='340' side='right'caption='[[2n3l]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
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<StructureSection load='2n3l' size='340' side='right'caption='[[2n3l]]' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[2n3l]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Plaf7 Plaf7]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2N3L OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2N3L FirstGlance]. <br>
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<table><tr><td colspan='2'>[[2n3l]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Plasmodium_falciparum_3D7 Plasmodium falciparum 3D7]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2N3L OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2N3L FirstGlance]. <br>
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</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PFE0865c ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=36329 PLAF7])</td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2n3l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2n3l OCA], [https://pdbe.org/2n3l PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2n3l RCSB], [https://www.ebi.ac.uk/pdbsum/2n3l PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2n3l ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2n3l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2n3l OCA], [https://pdbe.org/2n3l PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2n3l RCSB], [https://www.ebi.ac.uk/pdbsum/2n3l PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2n3l ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/Q8I3T5_PLAF7 Q8I3T5_PLAF7]
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Alternative splicing confers a complexity to the mRNA landscape of apicomplexans, resulting in a high proteomic diversity. The Plasmodium falciparum Ser/Arg-rich protein 1 (PfSR1) is the first protein to be confirmed as an alternative splicing factor in this class of parasitic protists [1]. A recent study [2] showed a purine bias in RNA binding among cognate RNA substrates of PfSR1. Here, we have investigated the role played by the amino-terminal RNA recognition motif (RRM1) of PfSR1 from the solution structure of its complex with ACAUCA RNA hexamer to understand how its mechanism of RNA recognition compares to human orthologs and to the C-terminal RRM. RNA binding by RRM1 is mediated through specific recognition of a cytosine base situated 5' of one or more pyrimidine bases by a conserved tyrosine residue on beta1 and a glutamate residue on the beta4 strand. Affinity is conferred through insertion of a 3' pyrimidine into a positively charged pocket. Retention of fast dynamics and ITC binding constants indicate the complex to be of moderate affinity. Using calorimetry and mapping of NMR chemical shift perturbations, we have also ascertained the purine preference of PfSR1 to be a property of the carboxy terminal pseudo-RRM (RRM2), which binds RNA non-canonically and with greater affinity compared to RRM1. Our findings show conclusive evidence of complementary RNA sequence recognition by the two RRMs, which may potentially aid PfSR1 in binding RNA with a high sequence specificity.
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The N-terminal RNA Recognition Motif of PfSR1 Confers Semi-specificity for Pyrimidines during RNA Recognition.,Ganguly AK, Verma G, Bhavesh NS J Mol Biol. 2019 Feb 1;431(3):498-510. doi: 10.1016/j.jmb.2018.11.020. Epub 2018 , Nov 28. PMID:30500338<ref>PMID:30500338</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2n3l" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Plaf7]]
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[[Category: Plasmodium falciparum 3D7]]
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[[Category: Bhavesh, N S]]
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[[Category: Bhavesh NS]]
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[[Category: Ganguly, A]]
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[[Category: Ganguly A]]
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[[Category: Verma, G]]
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[[Category: Verma G]]
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[[Category: Rna binding protein]]
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[[Category: Rna recognition motif]]
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[[Category: Serine/arginine-rich protein 1]]
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Current revision

Solution structure of RNA recognition motif-1 of Plasmodium falciparum serine/arginine-rich protein 1.

PDB ID 2n3l

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