6x5i
From Proteopedia
(Difference between revisions)
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==Cryo-EM of peptide-like filament of 1-KMe3== | ==Cryo-EM of peptide-like filament of 1-KMe3== | ||
| - | <StructureSection load='6x5i' size='340' side='right'caption='[[6x5i]]' scene=''> | + | <StructureSection load='6x5i' size='340' side='right'caption='[[6x5i]], [[Resolution|resolution]] 4.30Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6X5I OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6X5I FirstGlance]. <br> | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6X5I OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6X5I FirstGlance]. <br> | ||
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6x5i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6x5i OCA], [https://pdbe.org/6x5i PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6x5i RCSB], [https://www.ebi.ac.uk/pdbsum/6x5i PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6x5i ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 4.3Å</td></tr> |
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DPN:D-PHENYLALANINE'>DPN</scene>, <scene name='pdbligand=DTY:D-TYROSINE'>DTY</scene>, <scene name='pdbligand=M3L:N-TRIMETHYLLYSINE'>M3L</scene>, <scene name='pdbligand=UQ4:N-(7-nitro-2,1,3-benzoxadiazol-4-yl)-beta-alanine'>UQ4</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6x5i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6x5i OCA], [https://pdbe.org/6x5i PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6x5i RCSB], [https://www.ebi.ac.uk/pdbsum/6x5i PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6x5i ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Intracellular protein filaments are ubiquitous for cellular functions, but forming bona fide biomimetic intracellular filaments of small molecules in living cells remains elusive. Here, we report the in situ formation of self-limiting intracellular filaments of a small peptide via enzymatic morphological transition of a phosphorylated and trimethylated heterochiral tetrapeptide. Enzymatic dephosphorylation reduces repulsive intermolecular electrostatic interactions and converts the peptidic nanoparticles into filaments, which exhibit distinct types of cross-beta structures with either C7 or C2 symmetries, with the hydrophilic C-terminal residues at the periphery of the helix. Macromolecular crowding promotes the peptide filaments to form bundles, which extend from the plasma membrane to nuclear membrane and hardly interact with endogenous components, including cytoskeletons. Stereochemistry and post-translational modification (PTM) of peptides are critical for generating the intracellular bundles. This work may offer a way to gain lost functions or to provide molecular insights for understanding normal and aberrant intracellular filaments. | ||
| + | |||
| + | Artificial Intracellular Filaments.,Feng Z, Wang H, Wang F, Oh Y, Berciu C, Cui Q, Egelman EH, Xu B Cell Rep Phys Sci. 2020 Jul 22;1(7). doi: 10.1016/j.xcrp.2020.100085. Epub 2020, Jul 1. PMID:32776017<ref>PMID:32776017</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6x5i" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
Current revision
Cryo-EM of peptide-like filament of 1-KMe3
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Categories: Large Structures | Egelman EH | Feng Z | Wang F | Xu B
