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| | ==Solution structure of the ACBD3:PI4KB complex== | | ==Solution structure of the ACBD3:PI4KB complex== |
| - | <StructureSection load='2n73' size='340' side='right'caption='[[2n73]], [[NMR_Ensembles_of_Models | 45 NMR models]]' scene=''> | + | <StructureSection load='2n73' size='340' side='right'caption='[[2n73]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2n73]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2N73 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2N73 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2n73]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2N73 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2N73 FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2n72|2n72]]</div></td></tr> | + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2n73 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2n73 OCA], [https://pdbe.org/2n73 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2n73 RCSB], [https://www.ebi.ac.uk/pdbsum/2n73 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2n73 ProSAT]</span></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ACBD3, GCP60, GOCAP1, GOLPH1 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), PI4KB ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2n73 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2n73 OCA], [https://pdbe.org/2n73 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2n73 RCSB], [https://www.ebi.ac.uk/pdbsum/2n73 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2n73 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[https://www.uniprot.org/uniprot/GCP60_HUMAN GCP60_HUMAN]] Involved in the maintenance of Golgi structure by interacting with giantin, affecting protein transport between the endoplasmic reticulum and Golgi. Involved in hormone-induced steroid biosynthesis in testicular Leydig cells (By similarity).<ref>PMID:11590181</ref>
| + | [https://www.uniprot.org/uniprot/GCP60_HUMAN GCP60_HUMAN] Involved in the maintenance of Golgi structure by interacting with giantin, affecting protein transport between the endoplasmic reticulum and Golgi. Involved in hormone-induced steroid biosynthesis in testicular Leydig cells (By similarity).<ref>PMID:11590181</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Human]] | + | [[Category: Homo sapiens]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Hexnerova, R]] | + | [[Category: Hexnerova R]] |
| - | [[Category: Veverka, V]] | + | [[Category: Veverka V]] |
| - | [[Category: Kinase]]
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| - | [[Category: Transferase-transferase regulator complex]]
| + | |
| Structural highlights
Function
GCP60_HUMAN Involved in the maintenance of Golgi structure by interacting with giantin, affecting protein transport between the endoplasmic reticulum and Golgi. Involved in hormone-induced steroid biosynthesis in testicular Leydig cells (By similarity).[1]
Publication Abstract from PubMed
Phosphatidylinositol 4-kinase beta (PI4KB) is one of four human PI4K enzymes that generate phosphatidylinositol 4-phosphate (PI4P), a minor but essential regulatory lipid found in all eukaryotic cells. To convert their lipid substrates, PI4Ks must be recruited to the correct membrane compartment. PI4KB is critical for the maintenance of the Golgi and trans Golgi network (TGN) PI4P pools, however, the actual targeting mechanism of PI4KB to the Golgi and TGN membranes is unknown. Here, we present an NMR structure of the complex of PI4KB and its interacting partner, Golgi adaptor protein acyl-coenzyme A binding domain containing protein 3 (ACBD3). We show that ACBD3 is capable of recruiting PI4KB to membranes both in vitro and in vivo, and that membrane recruitment of PI4KB by ACBD3 increases its enzymatic activity and that the ACBD3:PI4KB complex formation is essential for proper function of the Golgi.
Structural insights and in vitro reconstitution of membrane targeting and activation of human PI4KB by the ACBD3 protein.,Klima M, Toth DJ, Hexnerova R, Baumlova A, Chalupska D, Tykvart J, Rezabkova L, Sengupta N, Man P, Dubankova A, Humpolickova J, Nencka R, Veverka V, Balla T, Boura E Sci Rep. 2016 Mar 24;6:23641. doi: 10.1038/srep23641. PMID:27009356[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Sohda M, Misumi Y, Yamamoto A, Yano A, Nakamura N, Ikehara Y. Identification and characterization of a novel Golgi protein, GCP60, that interacts with the integral membrane protein giantin. J Biol Chem. 2001 Nov 30;276(48):45298-306. Epub 2001 Oct 5. PMID:11590181 doi:http://dx.doi.org/10.1074/jbc.M108961200
- ↑ Klima M, Toth DJ, Hexnerova R, Baumlova A, Chalupska D, Tykvart J, Rezabkova L, Sengupta N, Man P, Dubankova A, Humpolickova J, Nencka R, Veverka V, Balla T, Boura E. Structural insights and in vitro reconstitution of membrane targeting and activation of human PI4KB by the ACBD3 protein. Sci Rep. 2016 Mar 24;6:23641. doi: 10.1038/srep23641. PMID:27009356 doi:http://dx.doi.org/10.1038/srep23641
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