|
|
| Line 1: |
Line 1: |
| | | | |
| | ==NMR structure of Guanylyl Cyclase Activator Protein 1 (GCAP1) mutant V77E in a Ca2+-free/Mg2+-bound Activator State== | | ==NMR structure of Guanylyl Cyclase Activator Protein 1 (GCAP1) mutant V77E in a Ca2+-free/Mg2+-bound Activator State== |
| - | <StructureSection load='2na0' size='340' side='right'caption='[[2na0]], [[NMR_Ensembles_of_Models | 15 NMR models]]' scene=''> | + | <StructureSection load='2na0' size='340' side='right'caption='[[2na0]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2na0]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bovin Bovin]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NA0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2NA0 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2na0]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NA0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2NA0 FirstGlance]. <br> |
| - | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MYR:MYRISTIC+ACID'>MYR</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MYR:MYRISTIC+ACID'>MYR</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">GUCA1A, GCAP1, GUCA1 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9913 BOVIN])</td></tr>
| + | |
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2na0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2na0 OCA], [https://pdbe.org/2na0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2na0 RCSB], [https://www.ebi.ac.uk/pdbsum/2na0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2na0 ProSAT]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2na0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2na0 OCA], [https://pdbe.org/2na0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2na0 RCSB], [https://www.ebi.ac.uk/pdbsum/2na0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2na0 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[https://www.uniprot.org/uniprot/GUC1A_BOVIN GUC1A_BOVIN]] Stimulates guanylyl cyclase 1 (GC1) when free calcium ions concentration is low and inhibits GC1 when free calcium ions concentration is elevated. This Ca(2+)-sensitive regulation of GC is a key event in recovery of the dark state of rod photoreceptors following light exposure.
| + | [https://www.uniprot.org/uniprot/GUC1A_BOVIN GUC1A_BOVIN] Stimulates guanylyl cyclase 1 (GC1) when free calcium ions concentration is low and inhibits GC1 when free calcium ions concentration is elevated. This Ca(2+)-sensitive regulation of GC is a key event in recovery of the dark state of rod photoreceptors following light exposure. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
| Line 23: |
Line 22: |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Bovin]] | + | [[Category: Bos taurus]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Ames, J B]] | + | [[Category: Ames JB]] |
| - | [[Category: Lim, S]] | + | [[Category: Lim S]] |
| - | [[Category: Activator state]]
| + | |
| - | [[Category: Lyase activator]]
| + | |
| - | [[Category: Signal transduction]]
| + | |
| Structural highlights
Function
GUC1A_BOVIN Stimulates guanylyl cyclase 1 (GC1) when free calcium ions concentration is low and inhibits GC1 when free calcium ions concentration is elevated. This Ca(2+)-sensitive regulation of GC is a key event in recovery of the dark state of rod photoreceptors following light exposure.
Publication Abstract from PubMed
GCAP1, a member of the neuronal calcium sensor (NCS) subclass of the calmodulin superfamily, confers Ca2+-sensitive activation of retinal guanylyl cylcase 1 (RetGC1). We present NMR resonance assignments, residual dipolar coupling (RDC) data, functional analysis, and a structural model of GCAP1 mutant (GCAP1V77E) in the Ca2+-free/Mg2+-bound state. NMR chemical shifts and RDC data reveal Ca2+-dependent differences for residues 170-174. An NMR-derived model of GCAP1V77E contains Mg2+ bound at EF2, and looks similar to Ca2+ saturated GCAP1 (RMSD = 2.0 A). Ca2+-dependent structural differences occur in the fourth EF-hand (EF4) and adjacent helical region (residues 164-174 called the Ca2+-switch helix). Ca2+-induced shortening of the Ca2+-switch helix changes solvent accessibility of T171 and L174 that affects the domain interface. Although the Ca2+-switch helix is not part of the RetGC1 binding site, insertion of an extra Gly residue between S173 and L174 as well as deletion of R172, S173 or L174, all caused a decrease in Ca2+-binding affinity and abolished RetGC1 activation. We conclude that Ca2+-dependent conformational changes in the Ca2+ switch helix are important for activating RetGC1, and provide further support for a Ca2+-myristoyl tug mechanism.
Structure of Guanylyl Cyclase Activator Protein 1 (GCAP1) Mutant V77E in a Ca2+-free/Mg2+-bound Activator State.,Lim S, Peshenko IV, Olshevskaya EV, Dizhoor AM, Ames JB J Biol Chem. 2015 Dec 24. pii: jbc.M115.696161. PMID:26703466[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Lim S, Peshenko IV, Olshevskaya EV, Dizhoor AM, Ames JB. Structure of Guanylyl Cyclase Activator Protein 1 (GCAP1) Mutant V77E in a Ca2+-free/Mg2+-bound Activator State. J Biol Chem. 2015 Dec 24. pii: jbc.M115.696161. PMID:26703466 doi:http://dx.doi.org/10.1074/jbc.M115.696161
|
