6wqh
From Proteopedia
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| - | ==== | + | ==Molecular basis for the ATPase-powered substrate translocation by the Lon AAA+ protease== |
| - | <StructureSection load='6wqh' size='340' side='right'caption='[[6wqh]]' scene=''> | + | <StructureSection load='6wqh' size='340' side='right'caption='[[6wqh]], [[Resolution|resolution]] 3.60Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6wqh]] is a 7 chain structure with sequence from [https://en.wikipedia.org/wiki/Dictyostelium_discoideum Dictyostelium discoideum] and [https://en.wikipedia.org/wiki/Meiothermus_taiwanensis Meiothermus taiwanensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6WQH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6WQH FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6wqh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6wqh OCA], [https://pdbe.org/6wqh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6wqh RCSB], [https://www.ebi.ac.uk/pdbsum/6wqh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6wqh ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.6Å</td></tr> |
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=4KZ:N-[(1R)-1-(DIHYDROXYBORANYL)-2-PHENYLETHYL]-NALPHA-(PYRAZIN-2-YLCARBONYL)-L-PHENYLALANINAMIDE'>4KZ</scene>, <scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=AGS:PHOSPHOTHIOPHOSPHORIC+ACID-ADENYLATE+ESTER'>AGS</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6wqh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6wqh OCA], [https://pdbe.org/6wqh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6wqh RCSB], [https://www.ebi.ac.uk/pdbsum/6wqh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6wqh ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The Lon AAA+ (adenosine triphosphatases associated with diverse cellular activities) protease (LonA) converts ATP-fuelled conformational changes into sufficient mechanical force to drive translocation of a substrate into a hexameric proteolytic chamber. To understand the structural basis for the substrate translocation process, we determined the cryo-electron microscopy (cryo-EM) structure of Meiothermus taiwanensis LonA (MtaLonA) in a substrate-engaged state at 3.6 A resolution. Our data indicate that substrate interactions are mediated by the dual pore loops of the ATPase domains, organized in spiral staircase arrangement from four consecutive protomers in different ATP-binding and hydrolysis states. However, a closed AAA+ ring is maintained by two disengaged ADP-bound protomers transiting between the lowest and highest position. This structure reveals a processive rotary translocation mechanism mediated by LonA-specific nucleotide-dependent allosteric coordination among the ATPase domains, which is induced by substrate binding. | ||
| + | |||
| + | Molecular basis for ATPase-powered substrate translocation by the Lon AAA+ protease.,Li S, Hsieh KY, Su SC, Pintilie GD, Zhang K, Chang CI J Biol Chem. 2021 Oct;297(4):101239. doi: 10.1016/j.jbc.2021.101239. Epub 2021 , Sep 24. PMID:34563541<ref>PMID:34563541</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6wqh" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Dictyostelium discoideum]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: | + | [[Category: Meiothermus taiwanensis]] |
| + | [[Category: Chang C]] | ||
| + | [[Category: Chiu W]] | ||
| + | [[Category: Hsiehb K]] | ||
| + | [[Category: Li S]] | ||
| + | [[Category: Pintilie G]] | ||
| + | [[Category: Sub S]] | ||
| + | [[Category: Zhang K]] | ||
Current revision
Molecular basis for the ATPase-powered substrate translocation by the Lon AAA+ protease
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