6xbo
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==X-ray crystal structure of the mouse CMP-Sialic acid transporter in complex with 5-methyl CMP== | ==X-ray crystal structure of the mouse CMP-Sialic acid transporter in complex with 5-methyl CMP== | ||
| - | <StructureSection load='6xbo' size='340' side='right'caption='[[6xbo]]' scene=''> | + | <StructureSection load='6xbo' size='340' side='right'caption='[[6xbo]], [[Resolution|resolution]] 1.80Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6XBO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6XBO FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6xbo]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6XBO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6XBO FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6xbo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6xbo OCA], [https://pdbe.org/6xbo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6xbo RCSB], [https://www.ebi.ac.uk/pdbsum/6xbo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6xbo ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8Å</td></tr> |
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=2PE:NONAETHYLENE+GLYCOL'>2PE</scene>, <scene name='pdbligand=5MC:5-METHYLCYTIDINE-5-MONOPHOSPHATE'>5MC</scene>, <scene name='pdbligand=OLC:(2R)-2,3-DIHYDROXYPROPYL+(9Z)-OCTADEC-9-ENOATE'>OLC</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6xbo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6xbo OCA], [https://pdbe.org/6xbo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6xbo RCSB], [https://www.ebi.ac.uk/pdbsum/6xbo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6xbo ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/S35A1_MOUSE S35A1_MOUSE] Transport CMP-sialic acid from the cytosol into Golgi vesicles where glycosyltransferases function. | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Nucleotide-sugar transporters (NSTs) transport nucleotide-sugar conjugates into the Golgi lumen where they are then used in the synthesis of glycans. We previously reported crystal structures of a mammalian NST, the CMP-sialic acid transporter (CST) (Ahuja and Whorton 2019). These structures elucidated many aspects of substrate recognition, selectivity, and transport; however, one fundamental unaddressed question is how the transport activity of NSTs might be physiologically regulated as a means to produce the vast diversity of observed glycan structures. Here, we describe the discovery that an endogenous methylated form of cytidine monophosphate (m5CMP) binds and inhibits CST. The presence of m5CMP in cells results from the degradation of RNA that has had its cytosine bases post-transcriptionally methylated through epigenetic processes. Therefore, this work not only demonstrates that m5CMP represents a novel physiological regulator of CST, but it also establishes a link between epigenetic control of gene expression and regulation of glycosylation. | ||
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| + | Inhibition of CMP-sialic acid transport by endogenous 5-methyl CMP.,Ahuja S, Cahill J, Hartfield K, Whorton MR PLoS One. 2021 Jun 3;16(6):e0249905. doi: 10.1371/journal.pone.0249905., eCollection 2021. PMID:34081697<ref>PMID:34081697</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6xbo" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| + | [[Category: Mus musculus]] | ||
[[Category: Ahuja S]] | [[Category: Ahuja S]] | ||
[[Category: Whorton MR]] | [[Category: Whorton MR]] | ||
Current revision
X-ray crystal structure of the mouse CMP-Sialic acid transporter in complex with 5-methyl CMP
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