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7n4y
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 7n4y is ON HOLD Authors: Description: Category: Unreleased Structures) |
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| - | '''Unreleased structure''' | ||
| - | The | + | ==The structure of bovine thyroglobulin with iodinated tyrosines== |
| + | <StructureSection load='7n4y' size='340' side='right'caption='[[7n4y]], [[Resolution|resolution]] 2.61Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[7n4y]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_indicus_x_Bos_taurus Bos indicus x Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7N4Y OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7N4Y FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.61Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=T44:3,5,3,5-TETRAIODO-L-THYRONINE'>T44</scene>, <scene name='pdbligand=TYI:3,5-DIIODOTYROSINE'>TYI</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7n4y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7n4y OCA], [https://pdbe.org/7n4y PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7n4y RCSB], [https://www.ebi.ac.uk/pdbsum/7n4y PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7n4y ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/A0A4W2CHS8_BOBOX A0A4W2CHS8_BOBOX] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Thyroglobulin is a homodimeric glycoprotein that is essential for the generation of thyroid hormones in vertebrates. Upon secretion into the lumen of follicles in the thyroid gland, tyrosine residues within the protein become iodinated to produce monoiodotyrosine (MIT) and diiodotyrosine (DIT). A subset of evolutionarily conserved pairs of DIT (and MIT) residues can then engage in oxidative coupling reactions that yield either thyroxine (T4; produced from coupling of a DIT `acceptor' with a DIT `donor') or triiodothyronine (T3; produced from coupling of a DIT acceptor with an MIT donor). Although multiple iodotyrosine residues have been identified as potential donors and acceptors, the specificity and structural context of the pairings (i.e. which donor is paired with which acceptor) have remained unclear. Here, single-particle cryogenic electron microscopy (cryoEM) was used to generate a high-resolution reconstruction of bovine thyroglobulin (2.3 A resolution in the core region and 2.6 A overall), allowing the structural characterization of two post-reaction acceptor-donor pairs as well as tyrosine residues modified as MIT and DIT. A substantial spatial separation between donor Tyr149 and acceptor Tyr24 was observed, suggesting that for thyroxine synthesis significant peptide motion is required for coupling at the evolutionarily conserved thyroglobulin amino-terminus. | ||
| - | + | The structure of natively iodinated bovine thyroglobulin.,Kim K, Kopylov M, Bobe D, Kelley K, Eng ET, Arvan P, Clarke OB Acta Crystallogr D Struct Biol. 2021 Nov 1;77(Pt 11):1451-1459. doi:, 10.1107/S2059798321010056. Epub 2021 Oct 29. PMID:34726172<ref>PMID:34726172</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 7n4y" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Bos indicus x Bos taurus]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Clarke OB]] | ||
| + | [[Category: Kim K]] | ||
Current revision
The structure of bovine thyroglobulin with iodinated tyrosines
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