7n6b

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'''Unreleased structure'''
 
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The entry 7n6b is ON HOLD
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==Structure of MmpL3 reconstituted into lipid nanodisc in the TMM bound state==
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<StructureSection load='7n6b' size='340' side='right'caption='[[7n6b]], [[Resolution|resolution]] 2.66&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[7n6b]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycolicibacterium_smegmatis_MC2_155 Mycolicibacterium smegmatis MC2 155]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7N6B OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7N6B FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.66&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=0HJ:6-O-[(2S)-2-{(1S)-18-[(1R,2R)-2-hexylcyclopropyl]-1-hydroxyoctadecyl}tricosanoyl]-alpha-D-glucopyranosyl+alpha-D-glucopyranoside'>0HJ</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7n6b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7n6b OCA], [https://pdbe.org/7n6b PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7n6b RCSB], [https://www.ebi.ac.uk/pdbsum/7n6b PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7n6b ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/MMPL3_MYCS2 MMPL3_MYCS2] Transports trehalose monomycolate (TMM) to the cell wall (PubMed:22520756, PubMed:28698380, PubMed:31239378). Flips TMM across the inner membrane. Membrane potential is not required for this function (PubMed:28698380). Transports probably phosphatidylethanolamine (PE) as well. Binds specifically both TMM and PE, but not trehalose dimycolate (TDM). Binds also diacylglycerol (DAG) and other phospholipids, including phosphatidylglycerol (PG), phosphatidylinositol (PI), and cardiolipin (CDL) (PubMed:31113875). Contributes to membrane potential, cell wall composition, antibiotic susceptibility and fitness (PubMed:28703701).<ref>PMID:22520756</ref> <ref>PMID:28698380</ref> <ref>PMID:28703701</ref> <ref>PMID:31113875</ref> <ref>PMID:31239378</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The mycobacterial membrane protein large 3 (MmpL3) transporter is essential and required for shuttling the lipid trehalose monomycolate (TMM), a precursor of mycolic acid (MA)-containing trehalose dimycolate (TDM) and mycolyl arabinogalactan peptidoglycan (mAGP), in Mycobacterium species, including Mycobacterium tuberculosis and Mycobacterium smegmatis. However, the mechanism that MmpL3 uses to facilitate the transport of fatty acids and lipidic elements to the mycobacterial cell wall remains elusive. Here, we report 7 structures of the M. smegmatis MmpL3 transporter in its unbound state and in complex with trehalose 6-decanoate (T6D) or TMM using single-particle cryo-electron microscopy (cryo-EM) and X-ray crystallography. Combined with calculated results from molecular dynamics (MD) and target MD simulations, we reveal a lipid transport mechanism that involves a coupled movement of the periplasmic domain and transmembrane helices of the MmpL3 transporter that facilitates the shuttling of lipids to the mycobacterial cell wall.
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Authors: Su, C.C., Yu, E.
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Structures of the mycobacterial membrane protein MmpL3 reveal its mechanism of lipid transport.,Su CC, Klenotic PA, Cui M, Lyu M, Morgan CE, Yu EW PLoS Biol. 2021 Aug 12;19(8):e3001370. doi: 10.1371/journal.pbio.3001370., eCollection 2021 Aug. PMID:34383749<ref>PMID:34383749</ref>
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Description: Structure of MmpL3 reconstituted into lipid nanodisc in the TMM bound state
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[Category: Unreleased Structures]]
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</div>
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[[Category: Yu, E]]
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<div class="pdbe-citations 7n6b" style="background-color:#fffaf0;"></div>
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[[Category: Su, C.C]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Mycolicibacterium smegmatis MC2 155]]
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[[Category: Su CC]]
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[[Category: Yu E]]

Current revision

Structure of MmpL3 reconstituted into lipid nanodisc in the TMM bound state

PDB ID 7n6b

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