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| - | [[Image:1en1.gif|left|200px]] | |
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| - | <!--
| + | ==STRUCTURE OF THE HIV-1 MINUS STRAND PRIMER BINDING SITE== |
| - | The line below this paragraph, containing "STRUCTURE_1en1", creates the "Structure Box" on the page.
| + | <StructureSection load='1en1' size='340' side='right'caption='[[1en1]]' scene=''> |
| - | You may change the PDB parameter (which sets the PDB file loaded into the applet)
| + | == Structural highlights == |
| - | or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
| + | <table><tr><td colspan='2'>[[1en1]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EN1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1EN1 FirstGlance]. <br> |
| - | or leave the SCENE parameter empty for the default display.
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| - | -->
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1en1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1en1 OCA], [https://pdbe.org/1en1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1en1 RCSB], [https://www.ebi.ac.uk/pdbsum/1en1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1en1 ProSAT]</span></td></tr> |
| - | {{STRUCTURE_1en1| PDB=1en1 | SCENE= }}
| + | </table> |
| - | | + | __TOC__ |
| - | '''STRUCTURE OF THE HIV-1 MINUS STRAND PRIMER BINDING SITE'''
| + | </StructureSection> |
| - | | + | [[Category: Large Structures]] |
| - | | + | [[Category: Johnson PE]] |
| - | ==Overview== | + | [[Category: Levin JG]] |
| - | The HIV-1 nucleocapsid protein (NC) functions as a nucleic acid chaperone during the plus-strand transfer step in reverse transcription by facilitating annealing of the primer binding site (PBS) sequence in the short plus-strand strong-stop DNA fragment [(+) SSDNA] to a complementary site located near the 3' end of the minus-strand DNA [(-) PBS DNA]. To investigate the mechanism by which NC performs this function, we have prepared an 18-nucleotide (-) PBS DNA for nuclear magnetic resonance (NMR) based structural and NC binding studies. The (-) PBS DNA forms a stable hairpin (T(m) approximately 42 +/- 5 degrees C) that contains a five-residue loop and a bulged thymine in a guanosine-cytosine-rich stem. Addition of substoichiometric amounts of NC results in significant broadening and reductions in NMR signal intensities of the Watson-Crick base-paired imino protons and a reduction by 20 degrees C in the upper temperature at which the imino proton signals are detectable, consistent with destabilization of the structure. The results suggest that inefficient annealing in the absence of NC may be due to the intrinsic stability of an internal (-) PBS DNA hairpin and that NC facilitates strand transfer by destabilizing the hairpin and exposing stem nucleotides for base pairing with the PBS sequence in (+) SSDNA.
| + | [[Category: Summers MF]] |
| - | | + | [[Category: Turner RB]] |
| - | ==About this Structure==
| + | [[Category: Wu ZR]] |
| - | Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EN1 OCA]. | + | |
| - | | + | |
| - | ==Reference== | + | |
| - | A mechanism for plus-strand transfer enhancement by the HIV-1 nucleocapsid protein during reverse transcription., Johnson PE, Turner RB, Wu ZR, Hairston L, Guo J, Levin JG, Summers MF, Biochemistry. 2000 Aug 8;39(31):9084-91. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10924101 10924101]
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| - | [[Category: Johnson, P E.]] | + | |
| - | [[Category: Levin, J G.]] | + | |
| - | [[Category: Summers, M F.]] | + | |
| - | [[Category: Turner, R B.]] | + | |
| - | [[Category: Wu, Z R.]] | + | |
| - | [[Category: Bulge]] | + | |
| - | [[Category: Hairpin]] | + | |
| - | [[Category: Pentaloop]] | + | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 15:17:53 2008''
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