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| | ==Solution structure of the Pax NRPS docking domain PaxB NDD== | | ==Solution structure of the Pax NRPS docking domain PaxB NDD== |
| - | <StructureSection load='7b2f' size='340' side='right'caption='[[7b2f]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | + | <StructureSection load='7b2f' size='340' side='right'caption='[[7b2f]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[7b2f]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Xenorhabdus_cabanillasii_jm26 Xenorhabdus cabanillasii jm26]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7B2F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7B2F FirstGlance]. <br> | + | <table><tr><td colspan='2'>Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7B2F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7B2F FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">XCR1_1150011 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1427517 Xenorhabdus cabanillasii JM26])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7b2f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7b2f OCA], [https://pdbe.org/7b2f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7b2f RCSB], [https://www.ebi.ac.uk/pdbsum/7b2f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7b2f ProSAT]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7b2f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7b2f OCA], [https://pdbe.org/7b2f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7b2f RCSB], [https://www.ebi.ac.uk/pdbsum/7b2f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7b2f ProSAT]</span></td></tr> |
| | </table> | | </table> |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Xenorhabdus cabanillasii jm26]]
| + | [[Category: Bode HB]] |
| - | [[Category: Bode, H B]] | + | [[Category: Duchardt-Ferner E]] |
| - | [[Category: Duchardt-Ferner, E]] | + | [[Category: Sarawi S]] |
| - | [[Category: Sarawi, S]] | + | [[Category: Watzel J]] |
| - | [[Category: Watzel, J]] | + | [[Category: Woehnert J]] |
| - | [[Category: Woehnert, J]] | + | |
| - | [[Category: Protein]]
| + | |
| - | [[Category: Protein binding]]
| + | |
| Structural highlights
Publication Abstract from PubMed
Non-ribosomal peptide synthetases (NRPS) produce natural products from amino acid building blocks. They often consist of multiple polypeptide chains which assemble in a specific linear order via specialized N- and C-terminal docking domains ((N/C) DDs). Typically, docking domains function independently from other domains in NRPS assembly. Thus, docking domain replacements enable the assembly of "designer" NRPS from proteins that normally do not interact. The multiprotein "peptide-antimicrobial-Xenorhabdus" (PAX) peptide-producing PaxS NRPS is assembled from the three proteins PaxA, PaxB and PaxC. Herein, we show that the small (C) DD of PaxA cooperates with its preceding thiolation (T1 ) domain to bind the (N) DD of PaxB with very high affinity, establishing a structural and thermodynamical basis for this unprecedented docking interaction, and we test its functional importance in vivo in a truncated PaxS assembly line. Similar docking interactions are apparently present in other NRPS systems.
Cooperation between a T Domain and a Minimal C-Terminal Docking Domain to Enable Specific Assembly in a Multiprotein NRPS.,Watzel J, Duchardt-Ferner E, Sarawi S, Bode HB, Wohnert J Angew Chem Int Ed Engl. 2021 Jun 14;60(25):14171-14178. doi:, 10.1002/anie.202103498. Epub 2021 May 14. PMID:33876501[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Watzel J, Duchardt-Ferner E, Sarawi S, Bode HB, Wohnert J. Cooperation between a T Domain and a Minimal C-Terminal Docking Domain to Enable Specific Assembly in a Multiprotein NRPS. Angew Chem Int Ed Engl. 2021 Jun 14;60(25):14171-14178. doi:, 10.1002/anie.202103498. Epub 2021 May 14. PMID:33876501 doi:http://dx.doi.org/10.1002/anie.202103498
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