Journal:Acta Cryst D:S205979832100632X
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| - | - | + | <StructureSection load='' size='450' side='right' scene='88/886037/Cv/9' caption=''> |
| + | ===Structure of the unique tetrameric STENOFOLIA homeodomain bound with promoter DNA=== | ||
| + | <big>Prabhat Kumar Pathak, Fei Zhang, Shuxia Peng, Lifang Niu, Juhi Chaturvedi, Justin Yoshida Elliott, Yan Xiang, Million Tadege, Junpeng Deng</big> <ref>doi: 10.1107/S205979832100632X</ref> | ||
| + | <hr/> | ||
| + | <b>Molecular Tour</b><br> | ||
| + | Homeodomain (HD) containing transcription factors are one of the most important regulators of morphology and differentiation in fungi, animals and plants. Among different types of HD containing transcriptional regulators, the WUCHEL-RELATED HOMEOBOX (WOX) family is unique to plants and instructs plant growth and development. The Medicago truncatula STENOFOLIA (STF) is a master regulator of leaf blade lateral development. Here, we report the crystal structure of the HD of STF in complex with its promoter DNA at 2.1 Å resolution. <scene name='88/886037/Cv/4'>STF-HD dimer</scene> (HDA in green, HDB in yellow) bound on a 22-bps DNA (color in magenta and alice blue for each strand respectively) in the ASU. <scene name='88/886037/Cv/8'>STF-HD binds DNA as a unique unprecedented tetramer</scene>, enclosing nearly entire bound DNA surface. Depicted are four STF-HDs (HDA' in blue and HDB' in wheat) bound on the DNA. HDA/HDA' and HDB/HDB' are crystallographic symmetry mates, respectively. A second DNA molecule in crystal packing is shown (DNAsym, colored in light pink and teal) forming a pseudo-continuous helix. <scene name='88/886037/Cv/13'>The electropotential of STF-HD tetramer</scene> is shown in sacefill representaion. The bound dsDNA is shown as cartoons. Notice nearly the entire DNA surface is clamped by the protein. Although the target promoter DNA sequence contains a mixture of 'TGA' and 'TAAT' motifs, STF-HD specifically binds 'TGA'. | ||
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| + | Major groove DNA base recognitions: | ||
| + | *<scene name='88/886037/Cv/28'>'TGA', DNA chain D</scene> (in cyan). | ||
| + | *<scene name='88/886037/Cv/29'>'TGA' recognition by STF-HDA helix alpha3</scene> (im green). | ||
| + | *<scene name='88/886037/Cv1/6'>'TGA', DNA chain C</scene> (in hot pink). | ||
| + | *<scene name='88/886037/Cv1/5'>'TGA' recognition by STF-HDB helix alpha3</scene> (in yellow). | ||
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| + | When compared to DNA recognition by other HDs, the 'base reader' helix alpha 3 in STF-HD not only specifies DNA sequence in the major groove of DNA, but also interacts with DNA from the minor groove providing important interactions: | ||
| + | *<scene name='88/886037/Cv2/9'>DNA minor groove interactions with HDA' helix alpha3</scene>. | ||
| + | *<scene name='88/886037/Cv/38'>DNA minor groove interactions with HDB' helix alpha3</scene>. | ||
| + | Our structure based mutagenesis and functional studies in planta support the structural observations. The findings from this study provide new insights into the DNA binding mechanisms of plant specific HDs from WOX family or transcription factors. | ||
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| + | '''PDB reference:''' STENOFOLIA homeodomain, [[6wig]] | ||
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| + | <b>References</b><br> | ||
| + | <references/> | ||
| + | </StructureSection> | ||
| + | __NOEDITSECTION__ | ||
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