Journal:Acta Cryst D:S205979832100632X
From Proteopedia
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Major groove DNA base recognitions: | Major groove DNA base recognitions: | ||
| - | *<scene name='88/886037/Cv/28'>'TGA'</scene> (in cyan). | + | *<scene name='88/886037/Cv/28'>'TGA', DNA chain D</scene> (in cyan). |
| - | *<scene name='88/886037/Cv/29'>'TGA' recognition by STF-HDA helix alpha3</scene> (green). | + | *<scene name='88/886037/Cv/29'>'TGA' recognition by STF-HDA helix alpha3</scene> (im green). |
| - | *<scene name='88/886037/Cv1/5'>'TGA' recognition by STF-HDB helix alpha3</scene> (yellow). | + | *<scene name='88/886037/Cv1/6'>'TGA', DNA chain C</scene> (in hot pink). |
| + | *<scene name='88/886037/Cv1/5'>'TGA' recognition by STF-HDB helix alpha3</scene> (in yellow). | ||
| - | When compared to DNA recognition by other HDs, the 'base reader' helix alpha 3 in STF-HD not only specifies DNA sequence in the major groove of DNA, but also interacts with DNA from the minor groove providing important interactions. Our structure based mutagenesis and functional studies in planta support the structural observations. The findings from this study provide new insights into the DNA binding mechanisms of plant specific HDs from WOX family or transcription factors. | + | When compared to DNA recognition by other HDs, the 'base reader' helix alpha 3 in STF-HD not only specifies DNA sequence in the major groove of DNA, but also interacts with DNA from the minor groove providing important interactions: |
| + | *<scene name='88/886037/Cv2/9'>DNA minor groove interactions with HDA' helix alpha3</scene>. | ||
| + | *<scene name='88/886037/Cv/38'>DNA minor groove interactions with HDB' helix alpha3</scene>. | ||
| + | Our structure based mutagenesis and functional studies in planta support the structural observations. The findings from this study provide new insights into the DNA binding mechanisms of plant specific HDs from WOX family or transcription factors. | ||
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| + | '''PDB reference:''' STENOFOLIA homeodomain, [[6wig]] | ||
<b>References</b><br> | <b>References</b><br> | ||
Current revision
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This page complements a publication in scientific journals and is one of the Proteopedia's Interactive 3D Complement pages. For aditional details please see I3DC.
