7oy9
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of GMP reductase from mycobacterium smegmatis.== | |
| + | <StructureSection load='7oy9' size='340' side='right'caption='[[7oy9]], [[Resolution|resolution]] 2.80Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[7oy9]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycolicibacterium_smegmatis Mycolicibacterium smegmatis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7OY9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7OY9 FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8Å</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7oy9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7oy9 OCA], [https://pdbe.org/7oy9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7oy9 RCSB], [https://www.ebi.ac.uk/pdbsum/7oy9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7oy9 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Mycobacteria express enzymes from both the de novo and purine-salvage pathways. However, the regulation of these processes and the roles of individual metabolic enzymes have not been sufficiently detailed. Both Mycobacterium tuberculosis (Mtb) and Mycobacterium smegmatis (Msm) possess three guaB genes, but information is only available on guaB2, which encodes an essential inosine 5'-monophosphate dehydrogenase (IMPDH) involved in de novo purine biosynthesis. This study shows that guaB1, annotated in databases as a putative IMPDH, encodes a guanosine 5'-monophosphate reductase (GMPR), which recycles guanosine monophosphate to inosine monophosphate within the purine-salvage pathway and contains a cystathionine-beta-synthase domain (CBS), which is essential for enzyme activity. GMPR activity is allosterically regulated by the ATP/GTP ratio in a pH-dependent manner. Bioinformatic analysis has indicated the presence of GMPRs containing CBS domains across the entire Actinobacteria phylum. | ||
| - | + | The mycobacterial guaB1 gene encodes a guanosine 5'-monophosphate reductase with a cystathionine-beta-synthase domain.,Knejzlik Z, Dolezal M, Herkommerova K, Clarova K, Klima M, Dedola M, Zbornikova E, Rejman D, Pichova I FEBS J. 2022 Mar 26. doi: 10.1111/febs.16448. PMID:35338694<ref>PMID:35338694</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 7oy9" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Mycolicibacterium smegmatis]] | ||
| + | [[Category: Dolezal M]] | ||
| + | [[Category: Klima M]] | ||
| + | [[Category: Pichova I]] | ||
Current revision
Crystal structure of GMP reductase from mycobacterium smegmatis.
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