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7e5t
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==Crystal structure of Fsa2== | ==Crystal structure of Fsa2== | ||
| - | <StructureSection load='7e5t' size='340' side='right'caption='[[7e5t]]' scene=''> | + | <StructureSection load='7e5t' size='340' side='right'caption='[[7e5t]], [[Resolution|resolution]] 2.17Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7E5T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7E5T FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7e5t]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Fusarium_sp._FN080326 Fusarium sp. FN080326]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7E5T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7E5T FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7e5t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7e5t OCA], [https://pdbe.org/7e5t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7e5t RCSB], [https://www.ebi.ac.uk/pdbsum/7e5t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7e5t ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1697752Å</td></tr> |
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=1PE:PENTAETHYLENE+GLYCOL'>1PE</scene>, <scene name='pdbligand=EOH:ETHANOL'>EOH</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7e5t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7e5t OCA], [https://pdbe.org/7e5t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7e5t RCSB], [https://www.ebi.ac.uk/pdbsum/7e5t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7e5t ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/FSA2_FUSSF FSA2_FUSSF] Diels-Alderase; part of the gene cluster that mediates the biosynthesis of equisetin and fusarisetin A, 2 trans-fused decalin-containing tetramic acids with antimicrobial activity (PubMed:25770422). The PKS module of fsa1 together with the enoylreductase fsa3 catalyze the formation of the polyketide unit which is then conjugated to L-serine by the condensation domain of the fsa1 NRPS module (PubMed:25770422). Activity of the Dieckmann cyclase domain (RED) results in release of the intermediate N-desmethylequisetin also called trichosetin (PubMed:25770422). Diels-Alderase fsa2 is involved in endo-selective Diels-Alder cycloaddition to form the decalin ring of equisetin (PubMed:25770422, PubMed:28401214). Subsequent N-methylation is carried out by fsa4 to give equisetin (PubMed:25770422). The enzymatic gene responsible for the conversion of equisetin to fusarisetin A has not been identified yet and is probably located outside of the fsa cluster (PubMed:28401214).<ref>PMID:25770422</ref> <ref>PMID:28401214</ref> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Fusarium sp. FN080326]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Fujiyama K]] | [[Category: Fujiyama K]] | ||
Current revision
Crystal structure of Fsa2
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