7f8d

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(New page: '''Unreleased structure''' The entry 7f8d is ON HOLD Authors: Shimozawa, Y., Himiyama, T., Nakamura, T., Nishiya, Y. Description: Malate Dehydrogenase from Geobacillus stearothermophil...)
Current revision (17:11, 29 November 2023) (edit) (undo)
 
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'''Unreleased structure'''
 
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The entry 7f8d is ON HOLD
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==Malate Dehydrogenase from Geobacillus stearothermophilus (gs-MDH) G218Y mutant==
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<StructureSection load='7f8d' size='340' side='right'caption='[[7f8d]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[7f8d]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Geobacillus_stearothermophilus Geobacillus stearothermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7F8D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7F8D FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7f8d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7f8d OCA], [https://pdbe.org/7f8d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7f8d RCSB], [https://www.ebi.ac.uk/pdbsum/7f8d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7f8d ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/A0A143T1U9_GEOSE A0A143T1U9_GEOSE] Catalyzes the reversible oxidation of malate to oxaloacetate.[HAMAP-Rule:MF_00487]
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Malate dehydrogenase (MDH) catalyzes the reversible reduction of nicotinamide adenine dinucleotide from oxaloacetate to L-malate. MDH from moderate thermophilic Geobacillus stearothermophilus (gs-MDH) has high thermal stability and substrate specificity and is used as a diagnostic reagent. In this study, gs-MDH was engineered to increase its catalytic activity at low temperatures. Based on sequential and structural comparison with lactate dehydrogenase from G. stearothermophilus, we selected G218 as a mutation site to increase the loop flexibility pivotal for MDH catalysis. The G218 mutants showed significantly higher specific activities than the wild type at low temperatures and maintained thermal stability. The crystal structure of the G218Y mutant, which had the highest catalytic efficiency among all the G218 mutants, suggested that the flexibility of the mobile loop was successfully increased by the bulky side chain. Therefore, this study demonstrated the low-temperature adaptation of MDH by facilitating conformational changes during catalysis.
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Authors: Shimozawa, Y., Himiyama, T., Nakamura, T., Nishiya, Y.
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Increasing loop flexibility affords low-temperature adaptation of a moderate thermophilic malate dehydrogenase from Geobacillus stearothermophilus.,Shimozawa Y, Himiyama T, Nakamura T, Nishiya Y Protein Eng Des Sel. 2021 Feb 15;34. pii: 6433232. doi: 10.1093/protein/gzab026. PMID:34850194<ref>PMID:34850194</ref>
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Description: Malate Dehydrogenase from Geobacillus stearothermophilus (gs-MDH) G218Y mutant
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[Category: Unreleased Structures]]
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</div>
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[[Category: Shimozawa, Y]]
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<div class="pdbe-citations 7f8d" style="background-color:#fffaf0;"></div>
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[[Category: Himiyama, T]]
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[[Category: Nishiya, Y]]
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==See Also==
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[[Category: Nakamura, T]]
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*[[Malate Dehydrogenase 3D structures|Malate Dehydrogenase 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Geobacillus stearothermophilus]]
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[[Category: Large Structures]]
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[[Category: Himiyama T]]
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[[Category: Nakamura T]]
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[[Category: Nishiya Y]]
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[[Category: Shimozawa Y]]

Current revision

Malate Dehydrogenase from Geobacillus stearothermophilus (gs-MDH) G218Y mutant

PDB ID 7f8d

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