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6ka2

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Current revision

Crystal structure of a Thebaine synthase from Papaver somniferum in complex with TBN

Structural highlights

6ka2 is a 4 chain structure with sequence from Papaver somniferum. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.35Å
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

THS2_PAPSO Catalyzes the formation of thebaine from (7S)-salutaridinol 7-O-acetate at the expense of labile hydroxylated by-products, which are preferentially produced by spontaneous allylic elimination. No visible activity toward (7S)-salutaridinol (at pH 7).^[1]

Publication Abstract from PubMed

Thebaine synthase 2 (THS2) that can transform (7S)-salutaridinol 7-O-acetate to thebaine catalyzes the final step of thebaine biosynthesis in Papaver somniferum. Here, the crystal structures of THS2 and its complex with thebaine are reported, revealing the interaction network in the substrate-binding pocket. Subsequent docking and QM/MM studies was performed to further explore the catalytic mechanism of THS2. Our results suggest that T105 may abstract the proton of C4-OH from the substrate under the assistance of H89. The resulting C4-O(-) phenolate anion then attacks the nearby C5, and triggers intramolecular SN2' syn displacement with the elimination of O-acetyl group. Moreover, the latter SN2' reaction is the rate-determining step of the whole enzymatic reaction with an overall energy barrier of 18.8 kcal/mol. These findings are of pivotal importance to understand the mechanism of action of thebaine biosynthesis, and would guide enzyme engineering to enhance the production of opiate alkaloids via metabolic engineering.

Structural insights into thebaine synthase 2 catalysis.,Chen CC, Xue J, Peng W, Wang B, Zhang L, Liu W, Ko TP, Huang JW, Zhou S, Min J, Ma L, Dai L, Guo RT, Yu X Biochem Biophys Res Commun. 2020 Aug 20;529(2):156-161. doi:, 10.1016/j.bbrc.2020.05.199. Epub 2020 Jun 22. PMID:32703404^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Chen X, Hagel JM, Chang L, Tucker JE, Shiigi SA, Yelpaala Y, Chen HY, Estrada R, Colbeck J, Enquist-Newman M, Ibanez AB, Cottarel G, Vidanes GM, Facchini PJ. A pathogenesis-related 10 protein catalyzes the final step in thebaine biosynthesis. Nat Chem Biol. 2018 Jul;14(7):738-743. doi: 10.1038/s41589-018-0059-7. Epub 2018 , May 28. PMID:29807982 doi:http://dx.doi.org/10.1038/s41589-018-0059-7
↑ Chen CC, Xue J, Peng W, Wang B, Zhang L, Liu W, Ko TP, Huang JW, Zhou S, Min J, Ma L, Dai L, Guo RT, Yu X. Structural insights into thebaine synthase 2 catalysis. Biochem Biophys Res Commun. 2020 Aug 20;529(2):156-161. doi:, 10.1016/j.bbrc.2020.05.199. Epub 2020 Jun 22. PMID:32703404 doi:http://dx.doi.org/10.1016/j.bbrc.2020.05.199

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/6ka2"

@@ Line 3: / Line 3: @@
 <StructureSection load='6ka2' size='340' side='right'caption='[[6ka2]], [[Resolution|resolution]] 2.35&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[6ka2]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Opium_poppy Opium poppy]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6KA2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6KA2 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[6ka2]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Papaver_somniferum Papaver somniferum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6KA2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6KA2 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=D4R:(4R,7aR,12bS)-7,9-dimethoxy-3-methyl-2,4,7a,13-tetrahydro-1H-4,12-methanobenzofuro[3,2-e]isoquinoline'>D4R</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.35&#8491;</td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">THS2 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3469 Opium poppy])</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=D4R:(4R,7aR,12bS)-7,9-dimethoxy-3-methyl-2,4,7a,13-tetrahydro-1H-4,12-methanobenzofuro[3,2-e]isoquinoline'>D4R</scene></td></tr>
-<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Thebaine_synthase Thebaine synthase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.99.24 4.2.99.24] </span></td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6ka2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ka2 OCA], [https://pdbe.org/6ka2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6ka2 RCSB], [https://www.ebi.ac.uk/pdbsum/6ka2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6ka2 ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[https://www.uniprot.org/uniprot/THS2_PAPSO THS2_PAPSO]] Catalyzes the formation of thebaine from (7S)-salutaridinol 7-O-acetate at the expense of labile hydroxylated by-products, which are preferentially produced by spontaneous allylic elimination. No visible activity toward (7S)-salutaridinol (at pH 7).<ref>PMID:29807982</ref>
+[https://www.uniprot.org/uniprot/THS2_PAPSO THS2_PAPSO] Catalyzes the formation of thebaine from (7S)-salutaridinol 7-O-acetate at the expense of labile hydroxylated by-products, which are preferentially produced by spontaneous allylic elimination. No visible activity toward (7S)-salutaridinol (at pH 7).<ref>PMID:29807982</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 25: / Line 24: @@
 </StructureSection>
 [[Category: Large Structures]]
-[[Category: Opium poppy]]
+[[Category: Papaver somniferum]]
-[[Category: Thebaine synthase]]
+[[Category: Chen CC]]
-[[Category: Chen, C C]]
+[[Category: Guo RT]]
-[[Category: Guo, R T]]
+[[Category: Huang JW]]
-[[Category: Huang, J W]]
+[[Category: Liu WD]]
-[[Category: Liu, W D]]
+[[Category: Xue J]]
-[[Category: Xue, J]]
+[[Category: Yu XJ]]
-[[Category: Yu, X J]]
-[[Category: Biosynthetic protein]]
-[[Category: Crystallization]]
-[[Category: Synthase]]

6ka2

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Current revision

Crystal structure of a Thebaine synthase from Papaver somniferum in complex with TBN

Structural highlights

Function

Publication Abstract from PubMed

References

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