2rts

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==Chitin binding domain1==
==Chitin binding domain1==
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<StructureSection load='2rts' size='340' side='right'caption='[[2rts]], [[NMR_Ensembles_of_Models | 30 NMR models]]' scene=''>
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<StructureSection load='2rts' size='340' side='right'caption='[[2rts]]' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[2rts]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pyrfu Pyrfu]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RTS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2RTS FirstGlance]. <br>
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<table><tr><td colspan='2'>[[2rts]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pyrococcus_furiosus_DSM_3638 Pyrococcus furiosus DSM 3638]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RTS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2RTS FirstGlance]. <br>
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</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PF1234 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=186497 PYRFU])</td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2rts FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rts OCA], [https://pdbe.org/2rts PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2rts RCSB], [https://www.ebi.ac.uk/pdbsum/2rts PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2rts ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2rts FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rts OCA], [https://pdbe.org/2rts PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2rts RCSB], [https://www.ebi.ac.uk/pdbsum/2rts PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2rts ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/Q8U1H4_PYRFU Q8U1H4_PYRFU]
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A chitinase, from Pyrococcus furiosus, is a hyperthermophilic glycosidase that effectively hydrolyses both alpha and beta crystalline chitin. This chitinase has unique structural features; it contains two catalytic domains (AD1 and AD2) and two chitin-binding domains (ChBD1 and ChBD2). We have determined the structure of ChBD1, which significantly enhances the activity of the catalytic domains, by nuclear magnetic resonance spectroscopy. The overall structure of ChBD1 had a compact and globular architecture consisting of three anti-parallel beta-strands, similar to those of other proteins classified into carbohydrate-binding module (CBM) family 5. A mutagenesis experiment suggested three solvent-exposed aromatic residues (Tyr112, Trp113 and Tyr123) as the chitin-binding sites. The involvement of Tyr123 or the corresponding aromatic residues in other CBMs, has been demonstrated for the first time. This result indicates that the binding mode may be different from those of other chitin-binding domains in CBM family 5. In addition, the binding affinities of ChBD1 and ChBD2 were quite different, suggesting that the two ChBDs each play a different role in efficiently increasing the activities of AD1 and AD2.
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Solution structure of the chitin-binding domain 1 (ChBD1) of a hyperthermophilic chitinase from Pyrococcus furiosus.,Mine S, Nakamura T, Sato T, Ikegami T, Uegaki K J Biochem. 2014 Feb;155(2):115-22. doi: 10.1093/jb/mvt104. Epub 2013 Nov 21. PMID:24272751<ref>PMID:24272751</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2rts" style="background-color:#fffaf0;"></div>
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==See Also==
==See Also==
*[[Chitinase 3D structures|Chitinase 3D structures]]
*[[Chitinase 3D structures|Chitinase 3D structures]]
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== References ==
 
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<references/>
 
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Pyrfu]]
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[[Category: Pyrococcus furiosus DSM 3638]]
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[[Category: Uegaki, K]]
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[[Category: Uegaki K]]
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[[Category: Chitin binding domain]]
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[[Category: Hydrolase]]
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Current revision

Chitin binding domain1

PDB ID 2rts

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