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| | ==Solution structure of the isolated histone H2A-H2B heterodimer== | | ==Solution structure of the isolated histone H2A-H2B heterodimer== |
| - | <StructureSection load='2rvq' size='340' side='right'caption='[[2rvq]], [[NMR_Ensembles_of_Models | 10 NMR models]]' scene=''> | + | <StructureSection load='2rvq' size='340' side='right'caption='[[2rvq]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2rvq]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RVQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2RVQ FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2rvq]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RVQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2RVQ FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">HIST1H2AB, H2AFM, HIST1H2AE, H2AFA ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), HIST1H2BJ, H2BFR ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2rvq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rvq OCA], [https://pdbe.org/2rvq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2rvq RCSB], [https://www.ebi.ac.uk/pdbsum/2rvq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2rvq ProSAT]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2rvq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rvq OCA], [https://pdbe.org/2rvq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2rvq RCSB], [https://www.ebi.ac.uk/pdbsum/2rvq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2rvq ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[https://www.uniprot.org/uniprot/H2B1J_HUMAN H2B1J_HUMAN]] Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.<ref>PMID:11859126</ref> <ref>PMID:12860195</ref> <ref>PMID:15019208</ref> Has broad antibacterial activity. May contribute to the formation of the functional antimicrobial barrier of the colonic epithelium, and to the bactericidal activity of amniotic fluid.<ref>PMID:11859126</ref> <ref>PMID:12860195</ref> <ref>PMID:15019208</ref>
| + | [https://www.uniprot.org/uniprot/H2A1B_HUMAN H2A1B_HUMAN] |
| - | <div style="background-color:#fffaf0;">
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| - | == Publication Abstract from PubMed ==
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| - | During chromatin-regulated processes, the histone H2A-H2B heterodimer functions dynamically in and out of the nucleosome. Although detailed crystal structures of nucleosomes have been established, that of the isolated full-length H2A-H2B heterodimer has remained elusive. Here, we have determined the solution structure of human H2A-H2B by NMR coupled with CS-Rosetta. H2A and H2B each contain a histone fold, comprising four alpha-helices and two beta-strands (alpha1-beta1-alpha2-beta2-alpha3-alphaC), together with the long disordered N- and C-terminal H2A tails and the long N-terminal H2B tail. The N-terminal alphaN helix, C-terminal beta3 strand, and 310 helix of H2A observed in the H2A-H2B nucleosome structure are disordered in isolated H2A-H2B. In addition, the H2A alpha1 and H2B alphaC helices are not well fixed in the heterodimer, and the H2A and H2B tails are not completely random coils. Comparison of hydrogen-deuterium exchange, fast hydrogen exchange, and {(1)H}-(15)N hetero-nuclear NOE data with the CS-Rosetta structure indicates that there is some conformation in the H2A 310 helical and H2B Lys11 regions, while the repression domain of H2B (residues 27-34) exhibits an extended string-like structure. This first structure of the isolated H2A-H2B heterodimer provides insight into its dynamic functions in chromatin.
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| - | Solution structure of the isolated histone H2A-H2B heterodimer.,Moriwaki Y, Yamane T, Ohtomo H, Ikeguchi M, Kurita J, Sato M, Nagadoi A, Shimojo H, Nishimura Y Sci Rep. 2016 May 16;6:24999. doi: 10.1038/srep24999. PMID:27181506<ref>PMID:27181506</ref>
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| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| - | </div>
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| - | <div class="pdbe-citations 2rvq" style="background-color:#fffaf0;"></div>
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| - | == References ==
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| - | <references/>
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Human]] | + | [[Category: Homo sapiens]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Ikeguchi, M]] | + | [[Category: Ikeguchi M]] |
| - | [[Category: Kurita, J]] | + | [[Category: Kurita J]] |
| - | [[Category: Moriwaki, Y]] | + | [[Category: Moriwaki Y]] |
| - | [[Category: Nagadoi, A]] | + | [[Category: Nagadoi A]] |
| - | [[Category: Nishimura, Y]] | + | [[Category: Nishimura Y]] |
| - | [[Category: Ohtomo, H]] | + | [[Category: Ohtomo H]] |
| - | [[Category: Sato, M]] | + | [[Category: Sato M]] |
| - | [[Category: Shimojo, H]] | + | [[Category: Shimojo H]] |
| - | [[Category: Yamane, T]] | + | [[Category: Yamane T]] |
| - | [[Category: Cs-rosetta]]
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| - | [[Category: Dna binding protein]]
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| - | [[Category: H2a]]
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| - | [[Category: H2b]]
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| - | [[Category: Histone]]
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| - | [[Category: Nuclear protein-nuclear protein complex]]
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| - | [[Category: Nucleosome]]
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