1bc7

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
(New page: 200px<br /> <applet load="1bc7" size="450" color="white" frame="true" align="right" spinBox="true" caption="1bc7, resolution 2.01&Aring;" /> '''SERUM RESPONSE FACT...)
Current revision (06:35, 7 February 2024) (edit) (undo)
 
(15 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1bc7.gif|left|200px]]<br />
 
-
<applet load="1bc7" size="450" color="white" frame="true" align="right" spinBox="true"
 
-
caption="1bc7, resolution 2.01&Aring;" />
 
-
'''SERUM RESPONSE FACTOR ACCESSORY PROTEIN 1A (SAP-1)/DNA COMPLEX'''<br />
 
-
==Overview==
+
==SERUM RESPONSE FACTOR ACCESSORY PROTEIN 1A (SAP-1)/DNA COMPLEX==
-
SAP-1 is a member of the Ets transcription factors and cooperates with SRF, protein to activate transcription of the c-fos protooncogene. The crystal, structures of the conserved ETS domain of SAP-1 bound to DNA sequences, from the E74 and c-fos promoters reveal that a set of conserved residues, contact a GGA core DNA sequence. Discrimination for sequences outside this, core is mediated by DNA contacts from conserved and nonconserved protein, residues and sequence-dependent DNA structural properties characteristic, of A-form DNA structure. Comparison with the related PU.1/DNA and, GABPalpha/beta/DNA complexes provides general insights into DNA, discrimination between Ets proteins. Modeling studies of a SAP-1/SRF/DNA, complex suggest that SRF may modulate SAP-1 binding to DNA by interacting, with its ETS domain.
+
<StructureSection load='1bc7' size='340' side='right'caption='[[1bc7]], [[Resolution|resolution]] 2.01&Aring;' scene=''>
-
 
+
== Structural highlights ==
-
==About this Structure==
+
<table><tr><td colspan='2'>[[1bc7]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BC7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1BC7 FirstGlance]. <br>
-
1BC7 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1BC7 OCA].
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.01&#8491;</td></tr>
-
 
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1bc7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bc7 OCA], [https://pdbe.org/1bc7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1bc7 RCSB], [https://www.ebi.ac.uk/pdbsum/1bc7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1bc7 ProSAT]</span></td></tr>
-
==Reference==
+
</table>
-
Structures of SAP-1 bound to DNA targets from the E74 and c-fos promoters: insights into DNA sequence discrimination by Ets proteins., Mo Y, Vaessen B, Johnston K, Marmorstein R, Mol Cell. 1998 Aug;2(2):201-12. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9734357 9734357]
+
== Function ==
 +
[https://www.uniprot.org/uniprot/ELK4_HUMAN ELK4_HUMAN] Involved in both transcriptional activation and repression. Interaction with SIRT7 leads to recruitment and stabilization of SIRT7 at promoters, followed by deacetylation of histone H3 at 'Lys-18' (H3K18Ac) and subsequent transcription repression. Forms a ternary complex with the serum response factor (SRF). Requires DNA-bound SRF for ternary complex formation and makes extensive DNA contacts to the 5'side of SRF, but does not bind DNA autonomously.
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bc/1bc7_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1bc7 ConSurf].
 +
<div style="clear:both"></div>
 +
__TOC__
 +
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
-
[[Category: Single protein]]
+
[[Category: Large Structures]]
-
[[Category: Johnston, K.]]
+
[[Category: Johnston K]]
-
[[Category: Marmorstein, R.]]
+
[[Category: Marmorstein R]]
-
[[Category: Mo, Y.]]
+
[[Category: Mo Y]]
-
[[Category: Vaessen, B.]]
+
[[Category: Vaessen B]]
-
[[Category: complex (dna-binding protein/dna)]]
+
-
[[Category: crystal structure]]
+
-
[[Category: dna-binding domain]]
+
-
[[Category: dna-binding specificity]]
+
-
[[Category: ets domain]]
+
-
[[Category: winged helix-turn-helix]]
+
-
 
+
-
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 16:08:21 2007''
+

Current revision

SERUM RESPONSE FACTOR ACCESSORY PROTEIN 1A (SAP-1)/DNA COMPLEX

PDB ID 1bc7

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1bc7"
Personal tools