6xr1
From Proteopedia
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==Computationally designed right-handed alpha/alpha single-chain toroid with 9 repeats== | ==Computationally designed right-handed alpha/alpha single-chain toroid with 9 repeats== | ||
| - | <StructureSection load='6xr1' size='340' side='right'caption='[[6xr1]]' scene=''> | + | <StructureSection load='6xr1' size='340' side='right'caption='[[6xr1]], [[Resolution|resolution]] 2.10Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6XR1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6XR1 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6xr1]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6XR1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6XR1 FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6xr1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6xr1 OCA], [https://pdbe.org/6xr1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6xr1 RCSB], [https://www.ebi.ac.uk/pdbsum/6xr1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6xr1 ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1Å</td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6xr1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6xr1 OCA], [https://pdbe.org/6xr1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6xr1 RCSB], [https://www.ebi.ac.uk/pdbsum/6xr1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6xr1 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Circular tandem repeat proteins ('cTRPs') are de novo designed protein scaffolds (in this and prior studies, based on antiparallel two-helix bundles) that contain repeated protein sequences and structural motifs and form closed circular structures. They can display significant stability and solubility, a wide range of sizes, and are useful as protein display particles for biotechnology applications. However, cTRPs also demonstrate inefficient self-assembly from smaller subunits. In this study, we describe a new generation of cTRPs, with longer repeats and increased interaction surfaces, which enhanced the self-assembly of two significantly different sizes of homotrimeric constructs. Finally, we demonstrated functionalization of these constructs with (1) a hexameric array of peptide-binding SH2 domains, and (2) a trimeric array of anti-SARS CoV-2 VHH domains. The latter proved capable of sub-nanomolar binding affinities towards the viral receptor binding domain and potent viral neutralization function. | ||
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| + | Design of functionalised circular tandem repeat proteins with longer repeat topologies and enhanced subunit contact surfaces.,Hallinan JP, Doyle LA, Shen BW, Gewe MM, Takushi B, Kennedy MA, Friend D, Roberts JM, Bradley P, Stoddard BL Commun Biol. 2021 Oct 29;4(1):1240. doi: 10.1038/s42003-021-02766-y. PMID:34716407<ref>PMID:34716407</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6xr1" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| + | [[Category: Synthetic construct]] | ||
[[Category: Bradley P]] | [[Category: Bradley P]] | ||
[[Category: Doyle L]] | [[Category: Doyle L]] | ||
[[Category: Hallinan JP]] | [[Category: Hallinan JP]] | ||
[[Category: Stoddard BL]] | [[Category: Stoddard BL]] | ||
Current revision
Computationally designed right-handed alpha/alpha single-chain toroid with 9 repeats
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