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7l7v

From Proteopedia

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Current revision

Crystal structure of Arabidopsis NRG1.1 CC-R domain K94E/K96E/R99E/K100E/R103E/K106E/K110E mutant

Structural highlights

7l7v is a 6 chain structure with sequence from Arabidopsis thaliana. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.95Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

DRL42_ARATH Probable disease resistance protein.

Publication Abstract from PubMed

Plant nucleotide-binding leucine-rich repeat receptors (NLRs) regulate immunity and cell death. In Arabidopsis, a subfamily of "helper" NLRs are required by many "sensor" NLRs. Active NRG1.1 oligomerized, was enriched in plasma membrane puncta and conferred cytoplasmic Ca(2+) influx in plant and human cells. NRG1.1-dependent Ca(2+) influx and cell death were sensitive to Ca(2+) channel blockers and were suppressed by mutations impacting oligomerization or plasma membrane enrichment. Ca(2+) influx and cell death mediated by NRG1.1 and ACTIVATED DISEASE RESISTANCE 1 (ADR1), another "helper" NLR, required conserved negatively charged N-terminal residues. Whole-cell voltage-clamp recordings demonstrate that Arabidopsis "helper" NLRs form Ca(2+)-permeable cation channels to directly regulate cytoplasmic Ca(2+) levels and consequent cell death. Thus, "helper" NLRs transduce cell death signals directly.

Plant "helper" immune receptors are Ca(2+)-permeable nonselective cation channels.,Jacob P, Kim NH, Wu F, El-Kasmi F, Chi Y, Walton WG, Furzer OJ, Lietzan AD, Sunil S, Kempthorn K, Redinbo MR, Pei ZM, Wan L, Dangl JL Science. 2021 Jun 17. pii: science.abg7917. doi: 10.1126/science.abg7917. PMID:34140391^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Jacob P, Kim NH, Wu F, El-Kasmi F, Chi Y, Walton WG, Furzer OJ, Lietzan AD, Sunil S, Kempthorn K, Redinbo MR, Pei ZM, Wan L, Dangl JL. Plant "helper" immune receptors are Ca(2+)-permeable nonselective cation channels. Science. 2021 Jun 17. pii: science.abg7917. doi: 10.1126/science.abg7917. PMID:34140391 doi:http://dx.doi.org/10.1126/science.abg7917

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/7l7v"

@@ Line 3: / Line 3: @@
 <StructureSection load='7l7v' size='340' side='right'caption='[[7l7v]], [[Resolution|resolution]] 2.95&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[7l7v]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Arath Arath]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7L7V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7L7V FirstGlance]. <br>
+<table><tr><td colspan='2'>[[7l7v]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7L7V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7L7V FirstGlance]. <br>
-</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">At5g66900, MUD21.16 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 ARATH])</td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.95&#8491;</td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7l7v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7l7v OCA], [https://pdbe.org/7l7v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7l7v RCSB], [https://www.ebi.ac.uk/pdbsum/7l7v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7l7v ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[https://www.uniprot.org/uniprot/DRL42_ARATH DRL42_ARATH]] Probable disease resistance protein.
+[https://www.uniprot.org/uniprot/DRL42_ARATH DRL42_ARATH] Probable disease resistance protein.
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 22: / Line 22: @@
 __TOC__
 </StructureSection>
-[[Category: Arath]]
+[[Category: Arabidopsis thaliana]]
 [[Category: Large Structures]]
-[[Category: Dangl, J L]]
+[[Category: Dangl JL]]
-[[Category: Lietzan, A D]]
+[[Category: Lietzan AD]]
-[[Category: Redinbo, M R]]
+[[Category: Redinbo MR]]
-[[Category: Walton, W G]]
+[[Category: Walton WG]]
-[[Category: Wan, L]]
+[[Category: Wan L]]
-[[Category: 4-helix bundle]]
-[[Category: Calcium channel]]
-[[Category: Cell death]]
-[[Category: Immune system]]
-[[Category: Membrane pore]]

7l7v

From Proteopedia

Current revision

Crystal structure of Arabidopsis NRG1.1 CC-R domain K94E/K96E/R99E/K100E/R103E/K106E/K110E mutant

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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